Labshake search
Citations for Quantifoil Micro Tools :
1 - 29 of 29 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plus carbon sample (Quantifoil Cu300 R2/1 + 2 nm C) was blotted for 2 s with blot force +3 after a 30 s wait time after sample application and immediately plunge frozen in liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... covered with either 2 nm amorphous carbon (Quantifoil, R2/1+2 nm C) or graphene were used for sample preparation ...
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bioRxiv - Biochemistry 2021Quote: ... C-flat grids (Protochips, USA; 1.2/1.3 300 mesh) and UltrAuFoil (Quantifoil, Germany; 1.2/1.3 300 mesh) were negatively glow discharged using PELCO Easiglow (Ted Pella ...
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bioRxiv - Immunology 2021Quote: ... by shaking at 1200 rpm for 5 minutes at 37°C using a plate shaker (Quantifoil Instruments) after stimulating with collagen at a range of concentrations ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
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bioRxiv - Cell Biology 2022Quote: Cells were trypsinized and seeded onto 200 mesh gold holey carbon grids (R2/2-2 C, Quantifoil Micro Tools) placed in 3D printed grid holders (51 ...
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bioRxiv - Immunology 2023Quote: ... The plate was then mixed (200rpm; 37°C) for 20 minutes in the dark (BioShake iQ, Quantifoil Instruments GmbH). Samples were fixed with 1% formalin and run on the Cytek Aurora 5-laser flow cytometer (Cytek Biosciences) ...
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bioRxiv - Biophysics 2024Quote: ... Aliquots of 1.25 – 1.5 ul RyR1-nanodisc were applied onto each side of glow-discharged C-Flat - 2/1 - 3Au holey-carbon (Protochips, NC) or UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μl 60 μM H6-TtBac in Buffer C were added onto freshly glow-discharged Quantifoil Cu/Rh R2/2 holey carbon 200 mesh grids (Quantifoil). The grids were blotted for 2.5 s with a blotting force of −15 ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... 3.0 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 1 s at 100 % humidity ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 3 s at 75 % humidity ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μL sample was applied onto a freshly glow-discharged 200-mesh copper 2 nm C R1.2/1.3 grids (Quantifoil Micro Tools). The grids were blotted for 1 s and plunge-vitrified in liquid ethane ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (BioShake iQ; Quantifoil Instruments ...
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bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 μL of the sample was applied to glow-discharged R1.2/1.3 holey carbon grids (Quantifoil Au or C-flat Cu). The grids were blotted for 3.5 s at 4°C and 100% humidity ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (Quantifoil Instruments, Jena, Germany). Next ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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bioRxiv - Biophysics 2023Quote: ... The collagen fibrils were transferred to glow-discharged electron microscopy grid by peeling off the collagen gel drop surface with the grid (purchased from QuantiFoil, C support Cu400), which was left on the collagen surface between 1 to 12 hours ...
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bioRxiv - Molecular Biology 2021Quote: The purified GIP1-42–GIPR–Gs–Nb35 complex at a concentration of 6-7 mg/mL was mixed with 100 μM GIP1-42 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GIPR–Gs–Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 μM tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified non-acylated tirzepatide–GIPR–mini-Gs–Nb35 complex at a concentration of 14-16 mg/mL was mixed with 100 μM non-acylated tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GIPR–Gs–Nb35 complex at a concentration of 5-6 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GCGR–Gs–Nb35 complex at a concentration of 12-14 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of the purified complex at a concentration of 5.72 mg/mL was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au/C 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific ...