Labshake search
Citations for Quantifoil Micro Tools :
1 - 43 of 43 citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were grown for 24 h on holey carbon-covered gold EM grids (200 mesh, R2/2, Quantifoil), fed with oleic acid and induced with doxycycline for Fsp27-EGFP expression for an unrelated project ...
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bioRxiv - Biophysics 2023Quote: We applied 3 μl of purified MeFDH1 (0.5 mg/ml) to a 1.2/1.3 holey carbon 200 mesh grid (Quantifoil), blotted for 4 s in a 100% humidity environment at 12 °C and then froze the specimens by plunging them into liquid ethane using a Vitrobot Mark IV (FEI).
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bioRxiv - Biochemistry 2023Quote: ... 300-mesh R0.6/1.0 holey carbon copper grid (preprocessed with 0.1% poly L-lysine hydrobromide after glow-discharging) (Quantifoil), or an R1.2/1.3 amorphous nickel titanium alloy (ANTA ...
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bioRxiv - Cell Biology 2021Quote: HeLa Doxycycline-inducible stable cell lines were grown for approximately 48 h on holey carbon gold EM grids (200 mesh, R2/2, Quantifoil) prior to plunge freezing ...
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bioRxiv - Biophysics 2020Quote: ... and 3 μl of the diluted sample was applied onto glow-discharged R1.2/1.3 300 mesh UltrAuFoil gold grids (Quantifoil) for 30 s and then blotted for 5 s before plunge-freezing the grids into liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: Purified GABAAR was incubated with ~1 μM Megabody-2524 and 3.5 μl sample was applied to glow discharged 300 mesh 1.2/1.3 and 2/2 UltraAuFoil gold grids (Quantifoil) for 30 s then blotted for 5.5 s prior to plunge-freezing with liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... 3.5 μL of the mixture was applied onto a glow-discharged Quantifoil R1.2/1.3 400 mesh Au grid (Quantifoil), blotted for 4 s at room temperature (RT ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µL of the assembled filament were applied to glow-discharged R1.2/1.3 Quantifoil 200 Au mesh grids (Quantifoil) in a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biophysics 2022Quote: ... 4 µL of the freshly prepared mixture were applied to glow-discharged (Quorum GloQube) R1.2/1.4 Cu 200 (Quantifoil) grids ...
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bioRxiv - Microbiology 2022Quote: ... A 4 μL droplet of the sample was applied onto a R1.2/1.3 300-mesh copper holey carbon grid (Quantifoil), previously glow discharged for 30 s at 30 mA using a GloQube system (Quorum Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl of samples was frozen onto a glow discharged Quantifoil Gold R 1.2/1.3 300 mesh grid (Quantifoil). Samples were frozen under 100% humidity ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 μl drop was applied to a graphene-treated and glow-discharged holey carbon grid (R 1.2/1.3 Quantifoil) for the BsClpP pH 4.2 sample and negatively glow-discharged holey carbon grid (R 1.2/1.3 Quantifoil ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μl sample were adsorbed for 45 s on glow-discharged 400 mesh R1.2/R1.3 holey carbon grids (Quantifoil) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the protein complex was applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). For cryo-EM grids preparation of DHA-FFA4-miniGq complex ...
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bioRxiv - Plant Biology 2021Quote: 4 μl of GmQUAC1malate proteins (purified in 75 mM L-malate) were applied to newly glow-discharged holy carbon film grids (Au R1.2/1.3, 300 meshes, Quantifoil, Germany). The grids were blotted with force 3 and blotting time of 9.0 s at 100% humidity and 4°C and were vitrified by plunge freezing into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of purified PA7LF3 at a concentration of ∼0.06 mg/ml was applied onto freshly glow discharged grids (Quantifoil R 1.2/1.3 holey carbon with a 2 nm additional carbon support ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were diluted twofold with Buffer F and 3.5 µl of sample was immediately applied onto a glow-discharged 300-mesh gold Quantifoil R1.2/1.3 grid (Quantifoil Micro Tools). Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... 3 µl of DeCLIC sample was applied to a glow-discharged quantifoil 1.2/1.3 Cu 300 mesh grid (Quantifoil Micro Tools), which was then blotted for 1.5 s and plunge-frozen into liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... 3.0 μL of above-described samples or non-treated CVA9 virions as a control were applied to glow-discharged grids (Quantifoil R1.2/1.3 holey carbon on 300 copper mesh ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μL of selected elution fractions were applied to a 300-mesh holey carbon grid (Au 1.2/1.3, Quantifoil Micro Tools), blotted 2 s at 75 % humidity ...
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Biochemistry 2024Quote: ... using 3 µL of the samples applied to previously glow discharged R 2/1 Holey Carbon Cu 200 mesh EM grids (Quantifoil). Samples were 10 µM FtsZ with or without ZapD or mZapD at different concentrations specified in each case (0 – 60 µM) ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL of sample solution was applied to a Quantifoil holey carbon grid (R1.2/1.3, Mo 200 mesh, Quantifoil Micro Tools GmbH) at 4 °C with 100% humidity ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Microbiology 2023Quote: A 1.4 μl sample solution was applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Mo grid, Quantifoil Micro Tools, Germany), and the grid was plunge-frozen into liquid ethane by Vitrobot mark IV (Thermo Fisher Science ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μl of this sample were placed on a glow-discharged EM grid (lacey carbon films on Cu 200 mesh, Quantifoil Micro Tools), blotted from the back side for 3 s ...
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bioRxiv - Microbiology 2020Quote: Usually 5 μL of liquid culture (OD680 ~ 0.3) was pipetted onto holey carbon-coated copper grids (R2/1, 200 mesh, Quantifoil Micro Tools, Jena, Germany). The grids were blotted from the reverse side and immediately plunged into the liquid ethane at liquid nitrogen temperature atmosphere using a Vitrobot ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 μL of purified CB1-Gi complex at 12 mg/ml was applied on glow- discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh) at room temperature (for AM11517) ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of purified CB1-Gi complex at 15 mg/ml was applied on glow- discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Neuroscience 2023Quote: A volume of 3 µl purified complex at a concentration of 1.5 mg/ml was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Cu 200 mesh). The grids were blotted with a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Microbiology 2019Quote: ... Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 of th0 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a volume of 6-8 μL of each sample was applied onto a gold grid covered by a holey gold film (UltrAuFoil 2/1, Quantifoil Micro Tools GmbH, Jena, Germany). Excess of liquid was blotted automatically between two strips of filter paper or only from the backside of the Grid ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...