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Citations for Quantifoil Micro Tools :
1 - 31 of 31 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 2.7 μl of freshly purified sample was applied onto glow-discharged (30 s, 5 mA) grids (Quantifoil R 0.6/1 300 mesh Cu or Ultrafoil 0.6/1 300 mesh Au ...
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bioRxiv - Immunology 2022Quote: ... 2.2 µl of the purified mixture at concentration of 0.2 mg/mL was applied to glow discharged (40 s at 25mA) UltraAu grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μl sample were adsorbed for 45 s on glow-discharged 400 mesh R1.2/R1.3 holey carbon grids (Quantifoil) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Four microliters of purified TilA-TslA-TlaA1-TlaA2 complex at 0.5 mg ml-1 in Buffer B (Supplementary Table 5) was applied onto glow-discharged (60 s, 30 mA) 300 mesh R1.2/1.3 Au grids (Quantifoil). Grids were blotted for 3 s with blot force of +15 at 10°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Biochemistry 2023Quote: ... 3.5 μL of HTNV-L at 1 µM was deposited on glow-discharged (25 mA, 45 s) UltraAuFoil 300 mesh R1.2/1.3 grids (Quantifoil). The grid was blotted for 3s (blot force 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μL of sample were applied for 30 s to 1.2/1.3 holey carbon on 300 mesh copper grids (Quantifoil), followed by 5 s blotting with blot force 20 and plunge freezing in liquid ethane with a Vitrobot IV (FEI ...
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bioRxiv - Biochemistry 2020Quote: ... 3.5 μl of this solution was adsorbed onto glow-discharged (PELCO EasiGlow, 15 mA, 0.39 mBar, 30 s) carbon-coated grids (200-400 mesh copper, Quantifoil) for 1 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... the pelleted cells resuspended in 100 μl NaH2PO4-NaOH pH 7.5 and a 3 μl drop adsorbed for 20 s to a carbon-coated copper grid (300 mesh, Quantifoil) that had been glow-discharged for 30 s at a current of 25 mA ...
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bioRxiv - Microbiology 2022Quote: ... and then loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R1.2/1.3, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... of 0.5 mg/mL purified SARS-CoV-2 S protein were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R1.2/1.3, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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High-resolution model of Arabidopsis Photosystem II reveals the consequences of digitonin-extractionbioRxiv - Plant Biology 2021Quote: ... 4 μl concentrated sample was applied to glow-discharged (30 s at 50 mA) Quantifoil R 1.2/1.3 Cu 300 grids (Quantifoil Micro Tools) and grids were plunge frozen in liquid ethane using an FEI Vitrobot MkIV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots (3.3 μL) of the S/2G1 complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... the postfusion S-nanodisc sample at 1.5 mg/ml was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Microbiology 2019Quote: 3 µl of S-layer suspension were applied to glow-discharged 300 mesh copper Quantifoil grids (R2/2, Quantifoil, Jena, Germany), blotted for 3 – 5 seconds and rapidly injected into liquid ethane using a homemade plunge-freezer ...
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bioRxiv - Immunology 2022Quote: A 3.5 μL sample of concentrated pNO-decorated CLPs was applied to negatively glow discharged (25 mA, 40 s) R3.5/1 quantifoil copper grids (Quantifoil Micro Tools). The excess of solution was blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of the complex was applied to glow-discharged (20 mA, 30 s; Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 0 and plunge-frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... of purified proteins of SARS-CoV2-ST or SARS-CoV2-STN were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R2/1, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: 4 μL of C5-OmCI-RaCI-CirpT1 in PBS (0.3 mg/ml) was applied to freshly glow-discharged (20 s, 15 mA) carbon-coated 200-mesh Cu grids (Quantifoil, R1.2/1.3). Following incubation for 10 s ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biophysics 2021Quote: ... The SEC-purified complex was concentrated using a 50 kDa cut-off concentrator to 1.5 mg/ml and applied to glow-discharged (0.39 mbar, 15 mA, 30 s) holey carbon grids (QuantiFoil R1.2/1.3 Au 200 mesh). Surplus sample was blotted off for 2-4 seconds with zero blotting force in a controlled environment (4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Biophysics 2022Quote: ... for 25 s) grid (For GDN 150mM: UltrAuFoil 300 mesh R 1.2/1.3, other samples: gold holey carbon Quantifoil 300 mesh R 1.2/1.3). After a ∼5 s manual wait time ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL of the ATP13A2 sample were applied to a glow-discharged (PELCO easiGlow; 0.39 mBar, 25–30 mA, 40–45 s) gold holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh). The grid was blotted for 3–4 s and plunge-frozen in liquid-nitrogen-cooled liquid ethane using Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: Freshly purified ZorAB sample was concentrated to 2-3 mg/mL and 2.7 μL protein was applied onto the glow-discharged (30 s, 5 mA) grids (Quantifoil R0.6/1 300 mesh Au) and plunge-frozen into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biophysics 2024Quote: Three microliters of Kir2.1 R312H mutant form were placed on glow-discharged (25 s) holey carbon-coated grids (Quantifoil R1.2/1.3, Au 200 mesh), blotted for 3.0 s ...
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bioRxiv - Cell Biology 2019Quote: ... 4 µl of the solution was applied to a glow-discharged (30 s at −35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). After removing excess liquid by blotting the grid from the back side with Whatman filter paper No ...