Labshake search
Citations for Quantifoil Micro Tools :
301 - 350 of 606 citations for R 4 Benzyl 2 2 diphenylphosphino benzyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... For the 20S:AKIRIN2:IPO9 sample R3.5/1 200 mesh Cu prefloated with 2 nm Carbon (Quantifoil) were used for plunge freezing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three-hundred mesh R1.2/1.3 UltrAuFoil grids with a layer of 2 nm amorphous carbon (Quantifoil) were glow discharged in a Pelco easiGlow for 12 seconds under a 0.37 mBar vacuum with 25 mA current ...
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bioRxiv - Immunology 2022Quote: ... grids (Quantifoil R 1.2/1.3 300 mesh) were first glow discharged for 60 seconds at 15 mA with a Pelco easiGlow glow discharge unit and 4 μL LNP suspension was applied to the surface of the grid ...
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of the cells were applied to a glow-discharged 200 mesh holey carbon grid copper grid (Quantifoil R1.2/3) and vitrified in a liquid ethane on a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of the h20S-PA26E102A-Opt5 solution were applied to R2/2 400-mesh grids (Quantifoil) that had been plasma treated for 25 s using a glow discharger (Electron Microscopy Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL was applied to freshly glow-discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... 2 μl apoferritin was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil). Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL was applied to freshly glow discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Then 3 μl of the mixture was applied to a Quantifoil-Au-2/1-3C grid (Quantifoil) pretreated by glow-discharging at 20 mA for 1 min ...
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bioRxiv - Microbiology 2023Quote: ... The samples were applied to a Quantifoil R2/2 Cu 300 mesh grid (Quantifoil Micro Tools GmbH), which had been freshly glow-discharged for 60 s at 10 mA using PIB-10 (Vacuum Device) ...
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bioRxiv - Microbiology 2024Quote: ... 3 μL of sample was loaded onto glow discharged holey carbon grids (Quantifoil, 300 mesh, R1/2). Samples were blotted for 3s and plunge frozen in liquid ethane using a Leica EM GP2 to obtain vitrified ice for imaging ...
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bioRxiv - Microbiology 2024Quote: A purified filament fraction (2 µL) was applied to a copper grid (R1.2/1.3 300 mesh; Quantifoil) and glow-discharged for 10 s using a JEC-3000FC Auto Fine Coater (JEOL) ...
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bioRxiv - Biochemistry 2024Quote: Samples were concentrated to 0.2 mg/ml or 1 mg/ml and prepared either on copper grids with holey carbon support and an additional layer of 2 nm continuous carbon (R1.2,1.3, Quantifoil) or on UltAufoil grids (R0.6/1.0 ...
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bioRxiv - Neuroscience 2021Quote: ... 300 mesh R 1.2/1.3 gold grids (Quantifoil). Samples were plunge frozen in liquid nitrogen cooled liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... UltrAuFoil R 1.2/1.3 300 Au mesh (Quantifoil) grids were glow discharged with residual air for 30 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... UltrAuFoil R 1.2/1.3 300 Au mesh (Quantifoil) grids were glow discharged with residual air for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of samples were applied to glow-discharged Quantifoil R 0.6/1 and R 1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany), blotted for 3 s and then flash frozen in liquid ethane using the automatic plunge freezer EM GP2 at 100% relative humidity ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 µL sample was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil) coated in-house with graphene oxide55-57 ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of sample solution was applied onto a holey carbon grid (Quantifoil R2/1, Cu 300 mesh) covered with a thin amorphous carbon film ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μl of the resultant mixture was applied to glow-discharged (EasiGlow Pelco) R2/2 Quantifoil grids (Quantifoil). Excess sample was blotted away with a FEI Vitrobot (IV ...
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bioRxiv - Evolutionary Biology 2021Quote: Molybdenum R1.2/1.3 and R2/2 200-mesh grids with a holey carbon support film (Quantifoil, Jena, DE) were glow discharged for 40 s under high vacuum shortly before sample application ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu ...
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bioRxiv - Immunology 2021Quote: ... Then 3 μl of the protein mixture was applied to a Quantifoil-Cu-2/1-3C grid (Quantifoil) pretreated by glow-discharging at 15 mA for 1 min ...
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bioRxiv - Biophysics 2020Quote: 3 µl of the toroid suspension were deposited onto a glow-discharged holey carbon grid (Quantifoil R2/2), blotted with a filter paper for 4 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... K735 apo and K772-ANP datasets were collected on untreated carbon/copper grids (Quantifoil R2/2 300 mesh). The grids used for the other 15 datasets were gold grids (UltrAuFoil R2/2 200 mesh) ...
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bioRxiv - Biochemistry 2022Quote: ... were prepared by applying 3 μL of freshly prepared STC (1:2 dilution) to UltrAuFoil 300 mesh R1.2/1.3 holey grids (Quantifoil), glow-discharged for 60 s at 10 mA (Leica EM ACE200) ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2023Quote: ... Phage particles were negatively stained for 20 seconds with 2% uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded onto gold grids (either ‘UltrAuFoil’ 200 Au mesh, R2/2 gold film, or Quantifoil 300 Au mesh ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μl of KMN complex at 0.3 mg/ml supplemented with 0.003% Triton were applied to glow-discharged UltrAuFoil R2/2 grids (Quantifoil). Excess liquid was removed by blotting (3.5 s at blot force −3) ...
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bioRxiv - Plant Biology 2023Quote: ... H4 NCPs were applied to Quantifoil R2/1 200-mesh grids coated with 2 nm of carbon (Quantifoil). H4.VS were applied to unsupported Quantifoil R2/1 200-mesh grids (Quantifoil) ...
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bioRxiv - Biochemistry 2023Quote: ... Differentiated U937 cells became adhesive and were seeded onto gold London Finder grids (Au H2 R2/2, Quantifoil) coated with vitronectin (25 µg/mL in 1xPBS ...