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Citations for IDT DNA :
1 - 22 of 22 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: DNA sequences flanked by XhoI and NdeI restriction sites and encoding for N-terminal GST-tagged-SIX1 or N-terminal GST-tagged-SIX1-Q177R homeodomains were synthesized by Integrative DNA Technologies (IDT) as gBlocks (Supplemental Table 2) ...
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bioRxiv - Cell Biology 2020Quote: ... G525R mutant forms of the mouse estrogen receptor (Ert1 and Ert2) and hemagglutinin (HA) epitope-tagged Dre recombinase (HA-Dre) tiles were synthesized by Gibson DNA assembly (IDT DNA), confirmed by Sanger DNA sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... the full-length codon optimized gene with N-terminal 6x-His-sequnce and NdeI-XhoI sites was synthesized (IDT DNA Inc.) (Table S2) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA/RNA oligonucleotides (Supplementary File 2) were obtained from IDT DNA technologies.
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bioRxiv - Microbiology 2020Quote: All plasmids are listed in Table 2 and oligonucleotides purchased from IDT DNA or Fisher used for their construction are listed in Table 3 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... annotated in the viral genome (GenBank accession MN985325 (13)) and (14)) were human codon-optimized using GenSmart™ Codon Optimization and synthesized by IDT DNA Technologies as gBlocks ...
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bioRxiv - Developmental Biology 2023Quote: ... ordered at the 2 nmol or 10 nmol scales as Custom DsiRNAs with standard purification (IDT DNA Technologies), resuspended at 70-100 μM in 1X Bombyx injection buffer (pH 7.2 ...
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bioRxiv - Molecular Biology 2023Quote: ... crRNA and trRNA (1:1) were complexed using touchdown polymerase chain reaction (PCR) (IDT DNA Technologies), and Cas9 3NLS protein (IDT DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid pLN-PP1-HA3-loxP was further modified to target endogenous pfpp1 with HA3 and loxP by introduction of a 5’ homology region for the gene (HR1, 682 bp of genomic DNA sequence for exons 2 and 3) fused to a recodonized synthetic fragment (IDT DNA) for exons 4 and 5 ...
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bioRxiv - Genetics 2020Quote: ... The hybridization solution is as follows: 0.6ng/ml Cy5 labeled 2’-O-Me-(CCCCGG)5 RNA probe (IDT DNA Technologies, IA), 0.02% BSA ...
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bioRxiv - Microbiology 2021Quote: ... The relative quantities of envelope (E) gene were measured using SARS-CoV-2 (2019-nCoV) CDC qPCR Probe Assay (IDT DNA technologies). Relative quantities of E gene were normalised to GAPDH mRNA levels (Applied Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... The relative quantity of nucleocapsid (N) RNA was measured using a SARS-CoV-2 (2019-nCoV) CDC qPCR N1 and control RNAseP probe set (IDT DNA Technologies). qPCR reactions were performed in duplicates with Taqman Universal PCR mix (Thermo ...
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bioRxiv - Immunology 2020Quote: ... The relative quantities of nucleocapsid (N) gene were measured using SARS-CoV-2 (2019-nCoV) CDC qPCR Probe Assay (IDT DNA technologies).
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bioRxiv - Microbiology 2022Quote: ... The relative quantities of envelope (E) gene were measured using SARS-CoV-2 (2019-nCoV) CDC qPCR Probe Assay (IDT DNA technologies). Relative quantities of E gene were normalized to GAPDH mRNA levels (Applied Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... as previously described.10 Genetic correction of the pathological gene variant in the patient-derived iPSC line UMGi137-A clone 2 was performed using ribonucleoprotein-based CRISPR/Cas9 using crRNA/tracrRNA and Hifi SpCas9 (IDT DNA technologies) by targeting exon 15 of the LZTR1 gene ...
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bioRxiv - Neuroscience 2023Quote: ... cells were generated using the Alt-R CRISPR-Cas9 System (IDT DNA Technologies) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... Control cells were transfected with the nuclease duplex buffer from IDT DNA (USA) instead of crRNA.
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bioRxiv - Microbiology 2020Quote: ... a synthetic fragment for a recodonized 3’ sequence of exon 1 followed by the artificial loxPint (IDT DNA), and a PCR-amplified fragment of the 5’ end of pfpp1 exon 2 (601 bp) ...
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bioRxiv - Immunology 2024Quote: ... Pvrig KO NK cells were isolated from spleens of Pvrig KO mice and transfected with two sgRNAs targeting mouse Tigit or Cd96 (IDT DNA) using Cas9-gRNA RNP-directed gene deletion on day 5 ...
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bioRxiv - Molecular Biology 2022Quote: Electrophoretic mobility shift assays were performed with different competitor DNA (ssDNA-salmon sperm DNA) conditions than SELEX-seq using Fam-labled dsDNA probes were generated by annealing upper fam labeled oligos (IDT DNA, Supplementary table 1). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Primary probes for iterative smFISH were obtained as a pool that contained equimolar amounts of each of the 358 oligonucelotides from IDT DNA (Supplementary Table 1). Readout probes were obtained with 3’ amino moieties and coupled to either Texas Red or Cy5 and purifed by HPLC like smFISH probes ...