Labshake search
Citations for G-Biosciences :
1 - 22 of 22 citations for Recombinant Human 5' Nucleotidase Ecto CD73 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
-
bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
-
bioRxiv - Neuroscience 2022Quote: ... 5 μg of head lysate or 5 μg of Human Normal Brain GenLysate (G-Biosciences) was subjected to standard SDS-PAGE using a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: Purification of tagged-GRFT was achieved by using a zinc-chelating resin (binding capacity 20mg/mL) (G-Biosciences). A calculated amount of resin was added to a 0.8 mL Pierce centrifuge spin column based on the estimated total protein yield obtained by Western blot analysis ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and the supernatant was mixed in a 1:1 ratio with HIS-Binding/Wash Buffer (G-Biosciences, USA) and 50 µl HisTag Dynabeads (Thermo Fischer Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were then sonicated and cell lysate containing GST tagged anti-GFP nanobody was incubated with glutathione resin (G-Biosciences, U.S.A.) at 4°C for 1 h ...
-
bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges ...
-
bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
-
bioRxiv - Biochemistry 2022Quote: ... 5 mM EDTA (G-Biosciences, #015E-F), 1X ProteaseArrestTM (G-Biosciences ...
-
bioRxiv - Microbiology 2019Quote: ... The resin was then resuspended in 5 ml of Buffer A and transferred to a 5-ml gravity column (G-Biosciences, MO, USA). The resin was further washed with 20 ml of Buffer A ...
-
bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) at 1:10,000 dilutions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
-
bioRxiv - Microbiology 2021Quote: ... Resins were then transferred to a 5-ml gravity column (G-Biosciences) and further washed with 25 ml of Buffer A ...
-
bioRxiv - Cancer Biology 2023Quote: ... After blocking the membranes with 5% blotto (G-Biosciences Blot-Quikblocker # 786-011) in phosphate-buffered saline containing 0.1% (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... Cleared lysates were passed through a 5 ml gravity column (G-Biosciences, MO, USA) containing 1.5 ml of Ni2+-NTA agarose resin (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Cellular lysates were prepared by using lysis buffer (supplied in RnD Systems Proteome Profiler Human Phosphokinase Array Kit supplemented with 1X “Protease Arrest” and 2X “Phosphatase Arrest” (G-Biosciences)) ...
-
bioRxiv - Bioengineering 2019Quote: ... pH 8.0; 5 mM MgCl2, 25 mM CaCl2; 50 mM Na2-EDTA, pH 8.0; 1 x ProteaseArrest™ agent, G-Bioscience). Equivalents of each 50 OD units were 5x diluted in thylakoid buffer to a final volume of 1 mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked for >60 min at room temperature with gentle shaking in 5% BLOT-QuickBlocker (w/v) (G-Bioscience 786-011) in phosphate buffered saline with 0.05% Tween-20 v/v (PBS-T).
-
bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
-
bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
-
bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...