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Citations for G-Biosciences :
1 - 46 of 46 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
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bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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bioRxiv - Synthetic Biology 2022Quote: ... and the supernatant was mixed in a 1:1 ratio with HIS-Binding/Wash Buffer (G-Biosciences, USA) and 50 µl HisTag Dynabeads (Thermo Fischer Scientific ...
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bioRxiv - Bioengineering 2022Quote: Purification of tagged-GRFT was achieved by using a zinc-chelating resin (binding capacity 20mg/mL) (G-Biosciences). A calculated amount of resin was added to a 0.8 mL Pierce centrifuge spin column based on the estimated total protein yield obtained by Western blot analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then sonicated and cell lysate containing GST tagged anti-GFP nanobody was incubated with glutathione resin (G-Biosciences, U.S.A.) at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges ...
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bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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bioRxiv - Immunology 2022Quote: ... 1:1 Non Animal Protein-BLOCKER™ (G-Biosciences) in TBS was added for 1 hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The wells were blocked with 1:1 Non-Animal Protein-BLOCKER™ (G-Biosciences) in TBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (G-Biosciences), 0.5 mM methylisobutylxanthine ...
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bioRxiv - Microbiology 2024Quote: ... 1% bromophenol blue (G-Biosciences)) ...
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bioRxiv - Immunology 2021Quote: ... 1:1 NAP-Blocker (NAP [Non Animal Protein]-BLOCKER™, G-Biosciences cat # 786-190) in TBS was added for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... AceTau (1:500, #ITK0084, G-Biosciences), MC1 (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM PMSF (G-Biosciences, #786), and 0.1% Triton X-100 (AMRESCO ...
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bioRxiv - Genetics 2022Quote: ... + 1 μM dexamethasone (G-bioscience, API-04) + 0.5 mM isobutylmethylxanthine (Sigma ...
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bioRxiv - Genomics 2021Quote: ... in 1 ml lysis buffer (G-Biosciences) with 10 µl proteinase K and digested for 1 hour at 60°C with periodic mixing to keep spores suspended in the lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 1 × Mammalian ProteaseArrest (G-Biosciences), 1 mM phenylmethane sulfonyl fluoride ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM PMSF (G-Biosciences, 82021). The cell lysates were clarified by centrifugation at 13,000 g at 4°C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF) (786-055, G-Biosciences) for 10 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of nickel-chelating resin slurry (G-Biosciences) was added to the cleared lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Neuroscience 2021Quote: ... amplicons were loaded in a 1% low EEO agarose gel (G-BioSciences) and purified using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Systems Biology 2020Quote: ... we treated the 1-mL samples with zymolyase (786-036, G-Biosciences). Zymolyase lysed vegetative cells that formed from germinated spores ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM β-glycerophosphate) using Tube-o-dialyzer 15 kDa (G-Biosciences) at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CB-Protein Assay kit and MCP-1 ELISA kit from G-Biosciences, India ...
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bioRxiv - Immunology 2023Quote: ... in 1×Tris-buffered saline Tween-20 (TBST, G-Biosciences, Cat#R042), and analyzed with the indicated primary antibodies (biotinylated anti-H3K27Me3 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were dialyzed against water (1 kDa MWCO, G-Biosciences Tube-O-DIALYZER) and stored at -80 deg C prior to inositol quantification by the UCSD GlycoAnalytics Core.
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bioRxiv - Cell Biology 2019Quote: ... 1 mM PMSF and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Microbiology 2022Quote: ... 1% NP-40) supplemented with 50 μg/ml phenylmethylsulfonyl fluoride (PMSF; G-Biosciences) and cOmplete protease cocktail inhibitor (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg/ml lysozyme and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Neuroscience 2023Quote: ... pH=7.5) supplemented with 1:100 proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Microbiology 2020Quote: ... 68 mM NaCl and 1 mM CaCl2) using a Tube-O-Dialyzer Micro (G-Biosciences) with a 50 kDa MWCO ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by the anti-VirE2 polyclonal antibody (G-Biosciences, 1:10000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein sample was then transferred to midi-size 1 kDa MWCO Tube-O-Dialyzers (G-Biosciences) and first dialyzed against 100 volumes of simplified high-salt buffer with urea overnight ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by anti-VirE2 polyclonal primary antibody (G-Biosciences, 1:10 000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 1% Tx100) supplemented with the protease inhibitor cocktail Protease Arrest (G-Biosciences, St. Louis, MO, USA). Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... albicans was performed according to the protocol of the Fast Yeast Transformation Kit (G-Biosciences, Cat. GZ-1). Gene deletion was verified by colony PCR as described [24] ...
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bioRxiv - Immunology 2022Quote: ... Blocks were sectioned and stained with Hematoxylin solution Gill III (MilliporeSigma) and 1% Eosin Y Solution (G-Biosciences). For immunofluorescent staining ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... supernatant pH was adjusted to 8.5 by addition of 1/20 volume 1M Tris pH=9.0 (G-Biosciences, 786-476), and the supernatant was filtered by 0.45 μm filter prior to purification on CaptureSelect™ C-tagXL Columns ...
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bioRxiv - Bioengineering 2019Quote: ... pH 8.0; 5 mM MgCl2, 25 mM CaCl2; 50 mM Na2-EDTA, pH 8.0; 1 x ProteaseArrest™ agent, G-Bioscience). Equivalents of each 50 OD units were 5x diluted in thylakoid buffer to a final volume of 1 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 15:1 (see Supplementary Information, 2. for more details) and 10 µL silica magnetic beads (G-Biosciences, USA). The mixture was briefly vortexed and incubated for 20 minutes at room temperature while shaking at 1000 rpm to facilitate DNA binding ...
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bioRxiv - Genetics 2023Quote: ... Fixed cells were lysed in 3X volume of lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH 8.0, 1X protease inhibitor cocktail (G-Biosciences, 786-433). Cells were sonicated for 35 minutes using a Diagenode Bioruptor to shear chromatin to 200-500bp ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...