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Citations for G-Biosciences :
1 - 34 of 34 citations for 6 Chloro 5 fluoro 1H indole 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Sulfo SASD (Sulphosuccinimidyl-2-(p-azidosalicylamido) ethyl-1,3-dithiopropionate) was purchased from G-biosciences. Dibenzocyclooctyne (DBCO)-Sulpho-NHS and DBCO-Sulpho-Cyanine5 were purchased form click chemistry tools and Genabioscience companies ...
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bioRxiv - Microbiology 2023Quote: Protein estimation was done using Bicinchoninic Acid (BCA) Protein Assay kit (G-Biosciences). Cell lysate (25ul aliquot of each replicate ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μg of head lysate or 5 μg of Human Normal Brain GenLysate (G-Biosciences) was subjected to standard SDS-PAGE using a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM EDTA (G-Biosciences, #015E-F), 1X ProteaseArrestTM (G-Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration in BEV samples was determined using Bicinchoninic acid assay (BCA; 786-571; G-Biosciences). AEC samples were buffer exchanged with 1× PBS prior to BCA to remove excess NaCl and adjust pH to 7.4.
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bioRxiv - Biochemistry 2022Quote: ... The fractions were loaded onto a G-Sep™ 6-600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... The resin was then resuspended in 5 ml of Buffer A and transferred to a 5-ml gravity column (G-Biosciences, MO, USA). The resin was further washed with 20 ml of Buffer A ...
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bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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bioRxiv - Cancer Biology 2021Quote: The F(ab′)2 fragments were generated using trastuzumab and the F(ab′)2 fragmentation kit following the manufacturer’s instructions (G-Biosciences).
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentration in BALF was determined using a Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572).
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing RNase E were loaded onto a G-Sep™ 6-600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... and GH (following 6 hrs-food withdrawal) were measured using commercially available ELISA kits (TSH ELISA kit, G-Biosciences, Cat No ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration of the samples was measured by Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572). 2-mercaptoethanol was added to a final concentration of 1% (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... after inducing expression with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM Mg-ATP ...
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bioRxiv - Plant Biology 2022Quote: ... coli Rosetta cells after induction with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM ATP ...
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bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) at 1:10,000 dilutions ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
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bioRxiv - Microbiology 2021Quote: ... Resins were then transferred to a 5-ml gravity column (G-Biosciences) and further washed with 25 ml of Buffer A ...
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bioRxiv - Cell Biology 2019Quote: Cells were incubated in serum-free DMEM for 24 h and IL-6/8 levels determined in supernatants using the femtoELISA™ HRP Kit (G-Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... After blocking the membranes with 5% blotto (G-Biosciences Blot-Quikblocker # 786-011) in phosphate-buffered saline containing 0.1% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Protein concentration of EV samples was determined using a bicinchoninic acid (BCA) assay using the manufacturer’s protocol (785-571; G-Biosciences, St. Louis, MO, USA). Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429 ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates were passed through a 5 ml gravity column (G-Biosciences, MO, USA) containing 1.5 ml of Ni2+-NTA agarose resin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... then excised and placed individually in 2-mL centrifuge tubes (G-Biosciences, Cat. #C041-D) for DNA purification.
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bioRxiv - Bioengineering 2019Quote: ... pH 8.0; 5 mM MgCl2, 25 mM CaCl2; 50 mM Na2-EDTA, pH 8.0; 1 x ProteaseArrest™ agent, G-Bioscience). Equivalents of each 50 OD units were 5x diluted in thylakoid buffer to a final volume of 1 mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked for >60 min at room temperature with gentle shaking in 5% BLOT-QuickBlocker (w/v) (G-Bioscience 786-011) in phosphate buffered saline with 0.05% Tween-20 v/v (PBS-T).
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bioRxiv - Microbiology 2021Quote: ... monolayers were overlaid with 2 ml of a solution consisting of equal parts of 1.4% (w/v) Low Melt Agarose (G-BioSciences) and 2X MEM (Gibco ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we first added 200 µL hexane containing 300 ng/µL of each mbCHC in a 2-mL glass vial and then used a nitrogen evaporator (BT1603 G-Biosciences®) to evaporate the hexane with only mbCHCs precipitate at the bottom of the vial ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 15:1 (see Supplementary Information, 2. for more details) and 10 µL silica magnetic beads (G-Biosciences, USA). The mixture was briefly vortexed and incubated for 20 minutes at room temperature while shaking at 1000 rpm to facilitate DNA binding ...
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bioRxiv - Biochemistry 2022Quote: ... A final concentration of 2 mM CaCl2 was added to the protein eluate prior to incubation with Calmodulin agarose beads (G-Biosciences; Cat# 786-282) for 90 min at 4 °C ...