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Citations for SouthernBiotech :
1 - 36 of 36 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... IgM or IgA and p-nitrophenyl phosphate substrate (SouthernBiotech). The reaction was stopped by adding 1.5 N NaOH solution and optical density was measured at 405 nm.
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bioRxiv - Genomics 2024Quote: ... incubating overnight at 4°C in DAPI fluoromount-G (Southern Biotech) and then mounting and imaging on a confocal laser microscope (Leica Sp8).
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bioRxiv - Immunology 2019Quote: ... were coated overnight at 4°C with goat anti-mouse Ig (SouthernBiotech). After blocking with 2% BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... legs expressing fluorescent proteins were mounted in Fluoromount 50 (SouthernBiotech). For immunohistochemistry ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed and incubated at 4°C with goat anti-mouse Ig-PE (SouthernBiotech). Cells were washed and mean fluorescence intensity (MFI ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with polyclonal goat anti-human IgA (Southern Biotech, #C5213-R466) (2 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated them with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL). Slices containing biocytin filled astrocytes were fixed ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated the slice with DAPI Fluoromount G overnight at 4°C (Cat# 0100-20; SouthernBiotech, Birmingham, AL) to ensure the whole slice could be visualized under fluorescence microscopy to localize the biocytin-filled ...
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bioRxiv - Immunology 2021Quote: ... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
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bioRxiv - Immunology 2019Quote: ... Sera were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of NP15-BSA or goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Immunology 2019Quote: ... culture supernatants were incubated 2 h at 37°C on wells pre-coated with 5 μg/mL of goat-anti-mouse IgM (Southern Biotech) and saturated with PBS 2% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were stored at 4°C until mounting on non-gelatin-coated slides followed by cover of DAPI fluoromount (Southern Biotech) and cover-slipped ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Microbiology 2024Quote: ... Following additional washings and incubation at 37°C for 1 h with AP-conjugated anti-goat IgG secondary antibody (Southern Biotech), absorbance was read by addition of PNPP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the embryos were stained with 10nM DAPI in 70% glycerol in 1X PBS overnight at 4°C protected from light, washed in 1X SSCT (10 min/wash, twice) before mounting with Fluoromount G (Southern Biotech) on glass bottom dishes (Cellvis ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with primary antibodies overnight at 4 °C and during 2 hours at room temperature with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (SouthernBiotech, USA). Membranes were then revealed using G:BOX Chemi XL1.4 Fluorescent & Chemiluminescent Imaging System (Syngene ...
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bioRxiv - Biochemistry 2021Quote: ... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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bioRxiv - Microbiology 2020Quote: ... the wells were incubated for 2 h at 37°C with HRP-mouse anti-human IgG antibody (dilution 1:2,000) (Southern Biotech, Birmingham, AL) followed by the peroxidase substrate ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2 μg/mL of monkey cross-adsorbed goat anti-human IgG (Southern Biotech #2049-01) diluted in phosphate-buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with FACS buffer and incubated for 30 min at 4°C with 1:1000 diluted PE-conjugated goat F(ab)’2 anti-human IgG (Southern Biotech 2042-09) in the dark ...
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bioRxiv - Biophysics 2023Quote: ... at a 1:250 dilution and protein was detected with a HRP-conjugated goat anti-mouse antibody (Southern Biotech) using a 1:5000 dilution ...
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bioRxiv - Immunology 2019Quote: ... 96-well Immulon 4 HBX plates were coated overnight at 4°C with 5 mg/ml polyclonal goat-anti mouse κ (Southern Biotech; 1050-01). Nonspecific binding was blocked with 1% bovine serum albumin in PBS and samples were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... IgG bound to the rSARS-CoV-2 S protein was detected using a horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Southern Biotech) incubated for 1 hour ± 10 minutes at 24°C ± 2°C ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Biochemistry 2022Quote: ... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... Equal coating levels of native and denatured TG3 were confirmed by detection of the biotinylated proteins with alkaline phosphatase-conjugated streptavidin (Southern Biotech).
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were coated with 1 μg/ml recombinant SARS-CoV-2 S protein (43) and serially diluted serum samples were added and detected via anti-monkey IgG-HRP (Southern Biotech, Birmingham, AL). Plates were developed using a TMB substrate (source ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated with hPD-L1-His protein and anti-PD-L1 antibody and anti-mouse IgG specific HRP conjugated secondary antibodies (SouthernBiotech, Birmingham, AL, USA) were added ...