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Citations for Millipore Sigma :
1 - 18 of 18 citations for pcDNA5 FRT since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: AD-293 cells were seeded and transfected the next day with either pcDNA5/FRT/TO-N-FLAG-hBirA*-GEMC1/MCIDAS or pcDNA5/FRT/TO-N-FLAG-hBirA* using PEI (Sigma-Aldrich) ±50 μM biotin (IBA GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 1–2 μg of pcDNA5 FRT/TO plasmids using GeneJuice Transfection Reagent (Millipore) on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100 ...
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bioRxiv - Molecular Biology 2024Quote: ... incubating 500 ng of supercoiled plasmid DNA (pcDNA5 FRT/TO and pOG44) with 100 µM Mirin (Sigma-Aldrich), 3.4 µM doxorubicin (known to exhibit strong DNA intercalation ...
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bioRxiv - Cell Biology 2020Quote: ... Flp-IN T-REx HEK293T cells were co-transfected with the pcDNA5/FRT/TO vector containing the sequence of FLAGOCIAD2 and the pOG44 vector using GeneJuice (Novagen) as transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... and pcDNA5/FRT/TO/2K-NS4B-3xFLAG-APEX2 in 9:1 (w/w) ratio using GeneJuice transfecting agent (Sigma-Merck) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and pcDNA5/FRT/TO Myc SARS CoV2 N and N 2A was transfected into HEK293T cells using GeneJuice (Novagen, Darmstadt, Germany) following the manufacturer’s protocol (12 μg of DNA/sample in total) ...
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... FRTs were digested with collagenase IV (Sigma) at 37°C by GentleMACS (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: HeLa Flp-In T-REx cells were transfected with pcDNA5-2xFlag-His6-siinsEXOSC10 (WT or K583R) or pcDNA5-2xFlag-His6 (empty vector) plasmids using XtremeGene9® transfection reagent (Sigma Aldrich, St Louis, MO, USA) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: FRT cell lines were cultured in F12 Coon’s modification media (Sigma, F6636) supplemented with 10% FBS and 1% Penicillin-Streptomycin (PenStrep) ...
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bioRxiv - Molecular Biology 2024Quote: FRT cell lines were cultured in F12 Coon’s modification media (Sigma, F6636) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: hTERT RPE-1 FRT/TO cells were cultured in F12/DMEM (Sigma-Aldrich) medium supplemented with GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 FRT/TO cells were cultured in F12/DMEM (Sigma-Aldrich) medium supplemented with GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... hTERT RPE-1 FRT/TO (RRID:CVCL_VP32) and derived cell lines listed in were grown in DMEM/F12 (Sigma-Aldrich, D6421) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT host cells harboring the Flp-In™ T-REx system were maintained in culture medium (DMEM-high glucose (Sigma) medium ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... DnaC::mdoB::kanR frt)26 were incubated from glycerol stock in LB media supplemented with 50 µg/mL Kanamycin antibiotic (K1876, Sigma-Aldrich) in a shaking incubator at 30°C and 300 rpm overnight ...