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Citations for Millipore Sigma :
1 - 30 of 30 citations for pEGFP C2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The pEGFP-N1.αSyn was generated from the pEGFP-N1 empty vector (Novagen) as previously described27 ...
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bioRxiv - Bioengineering 2024Quote: Collagenase type II (C2-BIOC, Millipore Sigma) was rehydrated in 1x HBSS at a concentration of 10mg/ml and stored in 5 ml tubes at -20°C ...
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bioRxiv - Cell Biology 2022Quote: The pEGFP-NONO_WT or pEGFP-NONO_ΔRRM1 plasmid was transformed into competent Rosetta 2(DE3)pLysS Escherichia coli cells (Novagen, 71400) and plated on LB agar plates with selection for kanamycin and chloramphenicol ...
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bioRxiv - Cell Biology 2024Quote: ... 1.5 mg/mL type II collagenase (Sigma-Aldrich, #C2-22), and 30 U/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... and 2 μM FM4-64 (SynaptoRed C2, Sigma catalogue no S6689).
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bioRxiv - Developmental Biology 2022Quote: ... and collagenase II (0.2%, Sigma–Aldrich, C2-22-1G, Darmstadt, Germany) for 6 to 8 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... NBD-DDA and the membrane dye SynaptoRed C2 (Sigma-Aldrich, S6689) were added to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with 8 µM of SynaptoRed C2 (S6689, Sigma) for 3 h at 30°C with shaking ...
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bioRxiv - Bioengineering 2023Quote: ... xylose labeled at positions C1 and C2 (Sigma-Aldrich, 99% purity). Cultures were grown under agitation (300 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... and TRAMP-C2 was cultured in DMEM supplemented with 10% FBS (Sigma) and 10 nM dihydrotestosterone (Sigma ...
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bioRxiv - Pathology 2023Quote: ... cell membranes were stained with 5% SynaptoRed C2 (Sigma-Aldrich no. S6689) in ddH2O for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μM SynaptoRed™ C2 (1 mM stock in DMSO, Sigma-Aldrich) or 50 μM BFA (50 mM stock in DMSO/Ethanol ...
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bioRxiv - Cell Biology 2022Quote: ... and digested in 1 ml of 0.5% collagenase type II (Merck Millipore C2-22) for 45 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Tunicamycin and C2-ceramide (N-Acetyl-D-sphingosine) were obtained from Sigma-Aldrich (St. Luis, USA) and were dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Genomics 2022Quote: ... The tissue was finely minced using scissors and digested with Collagenase type II (Sigma-Aldrich, C2-22)in 3% BSA-HBSS buffer at 37°C for 45 minutes with gentle shaking ...
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bioRxiv - Genetics 2021Quote: ... ML-DmBG3-c2 cells were cultured in M3 + BPYE + 10% FBS with 10 µg/ml insulin (Sigma-Aldrich) while CME W1 Cl.8+ cells required M3 + 2% FBS + 5 µg/ml insulin + 2.5% fly extract containing medium ...
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bioRxiv - Cell Biology 2024Quote: ... Collagen type I (cat#-804622-20ML) and Collagenase-II solution (cat#-C2-22-1G) were purchased from Sigma. Matrigen softslip 24 well culture plates with removable coverslips coated with 1 and 50 kPa stiffness (SS24-COL-1-EA ...
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bioRxiv - Cell Biology 2020Quote: ... DNA constructs corresponding to the mature form of human UBTD1 were subcloned from pEGFP-N1 (Novagen) to a retroviral vector compatible plasmid (PPRIG ...
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bioRxiv - Microbiology 2024Quote: ... Equal cell numbers of mid-log phase p25.C2 and AMD290 were conjugated for 6 hours on 0.45 μm nitrocellulose filters (Millipore) overlaid on LB agar plates containing Diaminopimelic acid ...
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bioRxiv - Biochemistry 2022Quote: ... equal amounts of C2 and C0 TRAP1 were treated with or without 50 mM DTT (Sigma-Aldrich, Missouri, USA) to achieve total reduction ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions that contained the SsCBE2-C2 protein were collected and concentrated through an Amicon Ultra centrifugal filter (Millipore). Following that ...
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bioRxiv - Molecular Biology 2022Quote: ... clones NM_004323.2-506s1c1 (clone 506) and NM_004323.2-666s1c1 (clone 666) or pLKO.1 non-silencing control (clone control C2) respectively (Mission®; Sigma-Aldrich). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from the HEK293 parent cell line and the homozygous c.1625A>G knock-in CRISPR/Cas9 clones C2 and F4 using TriPure (Sigma). cDNA was synthesized using POLR3B exon 18-specific primer 5’-TGTTGTGTTCGTACAGTGCAATGT and the Superscript III First-strand synthesis system (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... Permeabilization of the eggshell by perm-1 RNAi was confirmed using BioTracker 640 Red C2 (FM4-64) dye (Sigma-Aldrich). Embryos were hand-dissected in simple embryonic culture buffer (0.5 μg/μL Inulin ...
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bioRxiv - Immunology 2021Quote: ... Coding sequences of β- and α- PTSN were cloned into pEGFP-C1 and p3xFlag-CMV-7.1 (Sigma-Aldrich). β- PTSN 4A mutation (residues K539 ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with the mammalian expression vector pcDNA4.0/TO_EGFP-tau or pEGFP-C1_MAP4 using the HP-Xtreme transfection reagent (Sigma-Aldrich, 636624400). The cells were imaged 24h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... only C501 and C527 of C2-TRAP1 variant) were blocked by incubating proteins with 10 mM N-ethylmaleimide (NEM, Sigma-Aldrich, Missouri, USA) for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... the cells were inoculated with 1.77*105 genome equivalents per well of HAdV-C2-dE3B-GFP in 10 μl of full medium using bovine serum albumin (BSA, cell-culture grade, Sigma-Aldrich, St. Louis, USA)-blocked small bore tubing cartridges ...
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bioRxiv - Biochemistry 2023Quote: ... was defined as the amount of enzyme required to hydrolyze the substrate to produce 1 mol of p-nitrophenol per min. p-Nitrophenyl acetate C2 (CAS No. 830-03-5) was from Sigma-Aldrich (St.Louis, MO, USA).
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bioRxiv - Genetics 2024Quote: ... Following immunostaining with anti-TNNT2 (Cardiac troponin T2; DSHB, RV-C2; Mouse; 1:50) or ACTA2 (SM α-actin; Sigma, A2547, mouse, 1:200) antibodies ...