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Citations for Millipore Sigma :
1 - 26 of 26 citations for DSPE Alkyne since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... β-GlcNAc- alkyne (Sigma). Purified protein containing pAzF was added at ∼ 1 mg/mL concentration ...
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bioRxiv - Biochemistry 2024Quote: ... biotin-PEG4-alkyne (Sigma), β-GlcNAc- alkyne (Sigma) ...
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bioRxiv - Bioengineering 2021Quote: ... Alkyne Tris(benzyltriazolylmethyl)amine (THPTA, Sigma-Aldrich, 95%), 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA ...
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bioRxiv - Immunology 2024Quote: ... 50 μM Fluor 488-alkyne (761621, Sigma, USA), 2.5 mM sodium ascorbate (A103539 ...
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bioRxiv - Biophysics 2023Quote: ... and 1 % (v/v) DSPE-PEG (2000)-biotin (Sigma Aldrich). 30 µl of this solution was carefully aspirated and spread onto a Polyvinyl alcohol (PVA ...
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bioRxiv - Cell Biology 2020Quote: ... of freshly prepared click chemistry reaction mixture containing the following: 5 μM IRDye 800CW Alkyne (929-60002, Li-COR, UK) or 200 μM mPEG5k-Alkyne (JKA3177, Sigma, UK), 4 mM CuSO4 (451657 ...
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bioRxiv - Neuroscience 2022Quote: ... Alkyne-functionalized ATTO590 dye (Sigma-Aldrich, catalog no. 93990) was used at a concentration of 5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... TAMRA-alkyne tag (1:5000; Sigma Aldrich, Cat# 900932), TCEP solution (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5µM Biotin-PEG4-alkyne (Sigma-Aldrich, Cat. No. 764213). The reactions were incubated at room temperature for 1.5Hr and then precipitated with TCA as described above to remove excess click chemistry reagents ...
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bioRxiv - Biochemistry 2022Quote: ... unclicked BODIPY-alkyne was dissolved in dimethyl sulfoxide (DMSO; Sigma, D5879) to a final concentration of 5 mM and they were both stored at −20°C ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Bioengineering 2021Quote: ... 2.31μmol of DSPE-PEG-G-TAxI and 5.80μmol of 7,8-DHF (Millipore Sigma, Burlington, MA, USA) were mixed in 12mL solvent with chloroform and ethyl acetate (5:1 v/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Bovine serum albumin (≥98% purity) and diazo biotin−alkyne were purchased from Sigma-Aldrich. Streptavidin agrose resin was purchased from Thermo Fisher.
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bioRxiv - Cancer Biology 2023Quote: ... Click chemistry reaction was performed with biotin-PEG4-alkyne (100 μM, Cat# 764213, Millipore Sigma), CuSO4 (1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Copper-catalyzed azide-alkyne cycloaddition (CuAAC) was performed by sequential addition of 1 mM TCEP (Sigma), 34mM tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine ...
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bioRxiv - Molecular Biology 2024Quote: ... Ten μg of proteins were treated with 10 μM tetramethylrhodamine (TAMRA)-alkyne (Sigma-Aldrich, MO, USA), 1 mM TCEP ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting wetcake of functionalized carbon nanotubes was then dispersed via water bath sonication in DSPE-PEG (MW = 2000, Laysan Bio) or by covalently binding mPEG (MW=5000, Sigma Aldrich) followed by further dispersal in DSPE-PEG ...
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bioRxiv - Neuroscience 2022Quote: ... Alkyne agarose (25 μl per sample) was washed with milli-Q and pre-blocked with 2% PVP-40 (Sigma) in buffer 1 (100 mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... via a copper-catalyzed azide-alkyne cycloaddition (CuAAC) in a freshly prepared mixture of CuSO4 (1 mM, Sigma-Aldrich), THPTA (0.5 mM ...
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bioRxiv - Physiology 2023Quote: ... 100 µM C16-alkyne was conjugated with fatty acid-free BSA (5% v/v) (Sigma-Aldrich, catalog no. A7030) in DMEM LG or HG media by sonication (15 min ...
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bioRxiv - Biophysics 2020Quote: ... 10 µL of the lipid mixture (98 mol% SOPC and 2 mol % DSPE-PEG2000) dissolved in chloroform was spread on indium tin oxide coated glass slides (Sigma Aldrich, France). In order to ensure complete evaporation of chloroform the slides were desiccated under vacuum overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM HEPES at pH 7.4) supplemented with PIC and 10 µL 100 mM (in methanol) biotin-PEG4-alkyne (Sigma-Aldrich) was added ...
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bioRxiv - Bioengineering 2023Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG2000, Laysan Lipids, Arab, AL) were dissolved in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The aliquots were then subjected to biotinylation by copper-catalyzed azide–alkyne cycloaddition by incubation of the RNA with a copper mixture (CuSO4, THPTA, Sodium ascorbate, all from Sigma-Aldrich) and biotin azide (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Biochemistry 2024Quote: Lipids were extracted and alkyne-modified lipids were reacted with 3-azido-7-hydoxycoumarin (Cat. No. 909513, Sigma-Aldrich, Oakville, ON, CA) and resolved on a TLC plate as previously described (50) ...