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Citations for Millipore Sigma :
1 - 48 of 48 citations for Complement anaphylatoxin C5a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... and complement C5a levels in serum were quantified by ELISA (Histamine kit: Enzo Life Sciences, Farmingdale, NY; Complement C3a kit: Quidel, San Diego, CA; Complement C5a kit: Sigma-Aldrich, St. Louis, MO). Spleen tissues were homogenized with a FastPrep-24 instrument in RIPA buffer supplemented with EGTA ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... 0.1 nM C5a (Sigma C-5788) in HEPES medium was added to the bottom compartment in a total volume of 600 μL ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... Complement binding changes were assessed by incubated cells in blocking buffer and 20% complement (guinea pig complement, MilliPore). H99 cells with either mAb or complement were placed on a shaker for 1 hr at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... complement component 3 (Sigma, GW20073F); and GAPDH (Cell Signaling Technology ...
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bioRxiv - Immunology 2021Quote: ... Complement reagent Serum (Sigma S1-100ml), Goat anti FH ...
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bioRxiv - Microbiology 2020Quote: ... Lyophilized human complement serum (Sigma, USA) was reconstituted in sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2019Quote: ... guinea pig complement serum (Sigma-Aldrich), and ∼ 5 × 108 CFU V ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... or human complement C1q (Sigma-Aldrich) were immobilized on a CM5 or C1 sensor chip (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... Complement was obtained from lyophilized human sera (Sigma) reconstituted in sterile water as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Complement factor H from human plasma (Sigma Aldrich) was used at a dilution of 1:10000 prior to Anti-Factor H goat antibody at a dilution of 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to guinea pig complement serum (Sigma) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: Lyophilized guinea pig complement (Sigma-Aldrich, 234395-5ML), was resuspended according to the manufacturer’s instructions (5 ml of ultra-pure DNase/RNase free DDW and stored in aliquots at –80°C).
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... The commercially available complement factor H (FH; C5813; Sigma), factor I (FI ...
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bioRxiv - Microbiology 2020Quote: ... Heat-inactivated plasma and exogenous guinea pig complement (EMD Millipore) were incubated with the target strain ...
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bioRxiv - Pathology 2019Quote: ... Complement 5b-9 (204903-1MG, 1:50; EMD Millipore Corp). Following PBS-Tween 20 0.05% (PBS-T ...
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bioRxiv - Pathology 2019Quote: ... Complement 5b-9 (204903-1MG, 1:50; EMD Millipore Corp); and anti-ZO-1 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... a control with the complement inhibitor polyanetholesulfonic acid (Sigma-Aldrich) was done at a final concentration of 100 µg/ml (Supplementary Fig ...
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bioRxiv - Microbiology 2024Quote: ... After sterile filtration 20% of complement-inactivated horse serum (Sigma) and 20 µM EDDHA were added and the solution was heated up to 50°C ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of serum were incubated with guinea-pig complement (Sigma) and the target strain ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in 20% guinea pig complement serum (Sigma-Aldrich) in KSOM at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in guinea pig complement (Sigma, 1639, Lot no. SLBX9353) diluted 1:3 with KSOM for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and 12.5 μl of baby rabbit complement (Sigma-Aldrich, St Louis, MO). The sera were heated to 56°C for 30 min to inactivate any endogenous complement ...
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bioRxiv - Developmental Biology 2023Quote: ... after which they were transferred to 20% guinea pig complement (Sigma Aldrich)31 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in guinea pig complement (Sigma, 1639, Lot no. SLBX9353) diluted with global® medium in a 1:3 ratio for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... washed thoroughly and then incubated with guinea pig complement serum (Sigma S-1639) for 5 min ...
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bioRxiv - Developmental Biology 2023Quote: ... incubation in complement solution (20% in TCM-199+10% FBS medium; 234395, Millipore). Lysed cells were removed mechanically by pipetting.
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bioRxiv - Microbiology 2020Quote: ... Inhibition of complement pathways was carried out by adding 12.5 mM EDTA (Sigma Aldrich), 12.5 mM EGTA/Mg2+ (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Heat inactivated 2-fold serial dilutions of serum were incubated with guinea pig complement (Sigma) and the target strain ...
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bioRxiv - Molecular Biology 2019Quote: ... META-enriched cultures were isolated via resistance to complement-mediated lysis in 20% human serum (Sigma) for 30min at 37°C and validated via sherp expression [21,23].
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bioRxiv - Immunology 2021Quote: ... were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... new medium (DMEM low glucose from Lonza, supplemented with 1% rabbit complement sera from Sigma-Aldrich) containing 10% sera derived from immunized or not with gPRP14 rabbits or mice was added ...
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bioRxiv - Plant Biology 2020Quote: ... a DNA oligonucleotide reverse complement to U6 was 3’-end-labelled with Digoxigening-11-ddUTP (Sigma, USA) using a Terminal Deoxynucleotidyl Transferase (TdT ...
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bioRxiv - Immunology 2022Quote: ... The diluted IgGs were then mixed with complement-preserved guinea pig serum (Sigma-Aldrich; final concentration 5%). Note that this concentration of guinea pig sera serum has been verified to not kill spirochetes in the absence of CspZ-YA antibodies (data not shown) ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus (GISAID accession EPI_ISL_406862) were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then labeled with a mouse monoclonal antibody to complement components C3b and iC3b (Clone 3E7; Sigma-Aldrich) diluted 1:100 in blocking solution at 4°C overnight on a rocker ...
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bioRxiv - Pathology 2020Quote: ... the suspension adjusted to 1 × 106 parasites/mL in PBS and was incubated with 20% human complement (Sigma Aldrich). After 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.3% Triton-X100 for staining with complement antibodies or 10% normal goat serum and 0.3% Triton-X 100 solution (Sigma) for 1 hr ...
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bioRxiv - Immunology 2021Quote: Focus reduction neutralization test (FRNT) in the presence of increasing concentrations [volume/volume] of complement from normal human serum (Sigma), including 0 ...
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bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... gonococci were incubated (5 min and in parallel with the infections outlined above) in SH- and antibiotic-free cell culture medium spiked with normal human complement (Sigma) to a final concentration of 6% ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 32P-labeled probes that reverse complement to the targeted miRNAs were hybridized with membrane in PerfectHyb(tm) Plus Hybridization Buffer (Sigma) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The heat inactivated sera were serially diluted in BSK-II media without rabbit sera followed by being mixed with complement-preserved sera from guinea pig (Sigma-Aldrich) or chicken (Biowest ...
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bioRxiv - Microbiology 2024Quote: ... the medium was heated to 50°C and mixed 50:50 with 2x agar (50°C) containing 20% complement-inactivated horse serum (10% final concentration) (Sigma-Aldrich) to provide an iron source with complexed iron ...