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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Erythromycin 90 95% Erythromycin A N N Dimethyl 13C2 ~90% 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Cortical synaptosomes were incubated for 90 min at 4 °C with gentle shaking on Nunc Lab-Tek 8-well chamber slides (Sigma-Aldrich) coated with poly-D-lysine (ThermoScientific) ...
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bioRxiv - Bioengineering 2019Quote: ... 5 U/mL of FXIII and 1 U/mL of Thrombin were combined in the presence of 10mM Ca2+ with the pelleted µgels and allowed to incubate at 37 °C for 90 minutes between two glass slides (1mm thickness) surface coated with sigmacote (Sigma-Aldrich). The mechanical testing on the hydrogel scaffolds was done using a 5500 series Instron ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... 7 and 14) were cryopreserved in freezing medium containing 90% fetal bovine serum (FBS; Hyclone Laboratories Inc.) and 10% DMSO (Sigma-Aldrich) and stored in liquid nitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 90 min with either horse-radish peroxidase (HRP) conjugated goat anti-rabbit IgG (1:10000; EMD Millipore) or HRP conjugated goat anti-mouse IgG (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were collected after incubation with trypsin solution and resuspended in freezing medium made up of 90% FBS and 10% DMSO (Sigma, D8418). The cell viability was measured based on trypan blue staining method on an automated cell counter ...
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bioRxiv - Microbiology 2021Quote: ... PCLS with ciliary activity of at least 80-90% were selected and washed twice with phosphate-buffered saline (PBS; Sigma-Aldrich). Afterwards ...
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bioRxiv - Genetics 2020Quote: ... dehydrated in ascending ethanol concentrations (15%, 30%, 50%, 70% and 90%) and stained with an alcoholic eosin solution (HT1101116-500ML, Sigma-Aldrich) for 2 min ...
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bioRxiv - Bioengineering 2022Quote: ... following a liquid deposition silanation technique.[38] Hydrogel precursor (90 µL) was placed between a Sigmacote (Sigma-Aldrich, St. Louis, MO)-treated glass microscope slide and a silanated glass coverslip ...
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bioRxiv - Molecular Biology 2022Quote: ... Prior to contact inhibition (90% confluency) cells were treated with various doses of BPS (Sigma-Aldrich, Oakville, Ontario, cat no: 103039), BPA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Inactivation of Reelin expression was induced at postnatal day (p)45-60 by daily intraperitoneal injections of tamoxifen dissolved in 10% alcohol-90% sunflower oil for 3 consecutive days (180mg/kg/day; Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: Protein samples of RIPA-soluble or RIPA-insoluble/urea-soluble fractions were separated by electrophoresis (120 V for 90 min) on a polyacrylamide gel containing 12% acrylamide in the presence of reducing agent (2-mercaptoethanol, Sigma #63689). After SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed in TB and exposed to a series of glycerol dilutions (25, 40, 55, 70, 85 and 90%; Sigma-Aldrich), immediately mounted on a 0.1% gelatin-coated slide surrounded by agar (4% ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... 50µg of each protein sample was run on a 10% SDS-PAGE gel followed with a 90V transfer at 4°C for 90 minutes to a PVDF membrane (Millipore #IPFL00010). Membranes were blocked for one hour with Intercept PBS blocking buffer (LiCor #927-40000 ...
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bioRxiv - Cell Biology 2023Quote: IMR-90 normal human fibroblasts (ATCC, CCL-186) and HEK293T cells were cultured in DMEM (Sigma-Aldrich, D5796; Wako, 044-29765) supplemented with 10% FBS (BioWest ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE (90 V, 30 min; 120 V, 1 h) and transferred to a Polyvinylidene Fluoride (PVDF, Millipore, IPVH00010) membrane (290 mA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Voltage-dependent K+ currents were measured from -70 to +90 mV with a 20-pA step in each recording and 4-AP (200 µM, Sigma-Aldrich) was used to block K+ currents ...
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bioRxiv - Biochemistry 2023Quote: ... MAs were liberated from the dried cell debris by heating at 90 °C in 40% (w/v) tetrabutylammonium hydroxide (TBAH; Sigma-Aldrich) for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... with vehicle (90% cyclodextrin, 10% ethanol), XBD-173 (10mg/kg twice daily), or etifoxine (50mg/kg once daily, saline once daily) (Sigma Aldrich) for 7 consecutive days ...
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bioRxiv - Cell Biology 2024Quote: ... the non-specific sites were saturated by incubation at room temperature with agitation for 90 minutes in a solution of 1X PBS/5% milk/0.2% Tween (TWEEN©20, Sigma-Aldrich). The membranes were then incubated overnight at 4°C in a humidity chamber with the primary antibody diluted in PBS 1X/milk 5%/Tween 0.2% ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the cell culture incubator at 37°C with humidified 5% CO2 and subcultured when they reached 80%–90% confluence using Trypsin (T2610-100ML, Sigma-Aldrich).
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bioRxiv - Bioengineering 2024Quote: Hydrogels for cell culture experiments were fabricated by placing 90-µL drops of precursor solution between a 3-(trimethoxysilyl)propyl acrylate (Sigma-Aldrich) silanated 18-mm glass coverslip and a glass slide treated with SigmaCote (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells (named T-ALL#2) were frozen and conserved in 90% Fetal Calf Serum (FCS) and 10% DMSO solution (D5879, Sigma Aldrich), or injected into CD42.1.2 C57BL/6 recipient mice ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) for 30 min at 20°C in the dark ...
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bioRxiv - Physiology 2022Quote: ... Lysate equivalent to 2 mg of tissue was transferred to a new 1.5 mL tube and 100 µL of extraction solvent containing 5 ug succinate-d4 (Sigma-Aldrich #293075) and 1 µg myristate-d27 (CDN Isotopes #D-1711 ...
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bioRxiv - Microbiology 2023Quote: ... time-course experiments were performed for trypsin where HIV pseudovirus particles were incubated with a final concentration of 100 ug/mL Trypsin-TPCK (Sigma-Aldrich) for 1 ...
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bioRxiv - Genomics 2022Quote: ... basilensis 4G11 RB-TnSeq library22 were grown in medium containing 100 ug/mL kanamycin sulfate (Millipore Sigma part number 60615-5G). Cultures were grown and incubated at 30°C shaking at 250 rpm in Ultrayield flasks ...
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bioRxiv - Microbiology 2022Quote: ... 5% lysed horse blood (defibrinated 50% stock, Hatunalab AB Cat. N° 139) and 20 mg/mL β-NAD (Sigma, Lot# SLCD5502). Prior to use the 50% horse blood stock was freeze thawed five times and clarified via centrifugation twice for 30 minutes at 18,000 rpm at 4 °C and then filtrated using 0.2 μm membrane filter ...
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bioRxiv - Microbiology 2019Quote: ... cells were transferred (2% inoculation) into 50 ml of T-R minimal medium supplemented with 0.5% (w/v) N-acetylglucosamine (Sigma-Aldrich #G4875) as a carbon source and incubated ~12 hr at room temperature and 150 rpm ...
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bioRxiv - Immunology 2021Quote: ... Monomers were loaded with 0.2 mg/ml peptide at 37°C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma-Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: Desiccated tissue sections mounted on ITO glass slides were sprayed using an HTX TM-Sprayer (HTX Technologies, LLC) with 10 mg/ml N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC, Sigma #222488) dissolved in 70%:30% methanol:water ...
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bioRxiv - Microbiology 2021Quote: ... cadavers or conidia were extracted in 2-mL vials with 500 µL n-hexane (for liquid chromatography LiChrosolv®, Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was chopped into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed and injected into mice ovary orthotopically (n=8) followed by Doxycycline (2mg/ml) and 10% (w/v) sucrose (Sigma-Aldrich) in drinking water after 1 weeks of cells inoculation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CFP was cut into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...
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bioRxiv - Biophysics 2023Quote: ... we weakened N-cadherin-mediated cell-cell adhesion of SMCs [49] by using the medium with the addition of mouse monoclonal anti-N-cadherin antibody (10 µg/mL, C3865, Sigma-Aldrich). The SMCs were cultured for 48 h on trough-shaped concavity arrays ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Microbiology 2023Quote: ... tubes were allowed to cool for 10 mins at room temperature and 2 mL of spectroscopy grade n-heptane (Sigma 1043660500) and 667 uL of sterile deionized water were added to each tube and vortexed for 3 mins ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Immunology 2024Quote: ... the apical surface was washed and transwells were inverted into fresh 24 well culture plates containing HBSS alone or HBSS containing 100 nM N-formyl-methionyl-leucyl-phenylalanine (fMLP; Sigma; uninfected positive control). Neutrophils were applied to the basolateral compartment and transmigration was allowed to proceed for 2h (H292 cells ...
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bioRxiv - Neuroscience 2024Quote: ... Lipids were extracted with 600 µl ice-cold methyl tert-butyl ether (MTBE, Sigma-Aldrich) and 150 µl methanol (VWR) ...
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bioRxiv - Plant Biology 2021Quote: ... 1X B vitamins (0.5 μg/mL nicotinic acid, 1 ug/mL thiamine, 0.5 μg/mL pyridoxine, and 0.1 μg/mL myo-inositol, Sigma), and 8 g/L Bacto Agar (Becton Dickinson) ...
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bioRxiv - Cancer Biology 2021Quote: ... dimethyl-α-ketoglutarate (Sigma), adenine (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... dimethyl amiloride (Sigma, A125), heparin (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... dimethyl sulfate (DMS, Sigma) was added to the medium at concentrations of 6 or 24 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Dimethyl malonate (Sigma 136441), Harzianopyridone (Santa Cruz Biotechnology ...