Labshake search
Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by an overnight incubation step at 4°C with primary antibodies: anti-Smchd1 antibody (1:250; HPA039441; Sigma Aldrich, Zwijndrecht, the Netherlands), anti-Emerin (1:200 ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 2 -4 hours at RT with the following antibodies at the following concentrations: rabbit Cone Arrestin (1:3000, Millipore Sigma, cat. #AB15282), chicken B-galactosidase (1:3000 ...
-
bioRxiv - Genetics 2020Quote: ... Bone tissue was cut into 2-mm pieces and placed in PBS buffer containing 4% paraformaldehyde (Schuchardt, Muenchen, Germany) and 1% glutaraldehyde (Merck, electron microscopy grade, Sigma-Aldrich, Czech Republic) for 1 h at RT and then 1 week at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
-
bioRxiv - Cell Biology 2020Quote: ... Non-expanded cells from the same experiment were stored in 1× PBS at 4 °C after fixation and were permeabilized with 0.1% Triton X-100 (Sigma-Aldrich, catalog no. T8787) in 1× PBS prior to antibody labeling ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in PBS containing 0.02% Triton X-100 and 2% NDS overnight at 4°C with primary antibodies: anti-ChAT (1:500, goat polyclonal, Millipore Cat# AB144P, RRID:AB_2079751) and anti-phosphorylated ribosomal protein S6 (p-rpS6240/244 ...
-
bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
-
bioRxiv - Synthetic Biology 2021Quote: ... non-fat milk in TBST buffer (Tris-buffered saline containing 0.05% Tween-20) overnight at 4 °C and incubated with anti-FLAG antibody (Sigma, F1804, 1:1000 dilution) or anti-HA antibody (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... and probed over-night at 4°C either in TBS-TM with a 1:1000 anti-Flag antibody (Sigma-Aldrich; catalogue No F3165), or with a 1:10000 anti-LpoA antibody (loading control 28 ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated in rabbit anti-choline acetyltransferase (ChAT, the synthetic enzyme for acetylcholine) for three days at 4°C (1:150; Cat#AB143, EMD Millipore, Billerica, MA). After incubation ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... blots were incubated overnight at 4 °C with the corresponding primary antibody: anti-phospho-histone 2AX (Ser139) (EMD Millipore, 05-636, 1:1000), anti-phospho-Akt (Cell Signaling ...
-
bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitation was performed at 4°C for 1 hr and 30 minutes with 200 μl α-FLAG M2 beads (Sigma, St. Louis, MO) followed by four 10-minute washes using AP buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... the antibody – lysate mixture was incubated at 4°C with rotation for 1 hr prior to the addition of protein A/G agarose beads (Millipore, 50 L/IP) and continued overnight incubation ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were fixed for 1 h at RT by addition of 26.6 μL 16% PFA and 4 μg/ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS using standard bore tubing cartridges ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set, and Sigma phosphatase inhibitor set), incubated on ice for 30 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... Each section was probed overnight at 4°C with an optimized concentration of the anti-CAV1 (Sigma-Aldrich®, catalog #HPA049326, 1:1000) and anti-CAV2 (Novus Biologicals ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Sigma-Aldrich, Taufkirchen, Germany) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were blocked with 5% normal donkey serum containing 0,3% Triton in PBS for 1 h at RT followed by incubation for two overnights at 4°C with mouse anti-acetylated α-tubulin (1:500, Sigma-Aldrich, T7451) and rabbit anti-βIII-tubulin (1:500 ...
-
bioRxiv - Microbiology 2020Quote: ... 2.04 × 108 Huh-7.5-Cas9 cells were transduced by spinoculation at 1,000 g x 1 h in media containing 4 µg/ml polybrene (Millipore, cat. #TR-1003-G) and 20 mM HEPES (Gibco ...
-
bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
-
bioRxiv - Immunology 2020Quote: ... To purify devil MHC-I molecules by immunoaffinity purification, devil cells were expanded (1×109 cells/replicate, n=4) using Corning® HYPERFlask® M cell culture vessels (Sigma-Aldrich), harvested and washed in 1X-PBS before being snap frozen in liquid nitrogen and stored at −80°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Tadpoles were exposed to 4’-OH-PPF ± 5 nM T3 (3,3′,5-triiodo-L-thyronine sodium salt (T3) (Sigma - CAS 55-06-1)).
-
bioRxiv - Genomics 2020Quote: Nuclei were isolated from fresh frozen pulverized postmortem brain tissue using sucrose gradient centrifugation (4°C, 25 000 rpm, 1h) and incubated with anti-NeuN-488 antibody (Millipore MAB377X, 1:1000) in PBS and BSA ...
-
bioRxiv - Immunology 2020Quote: ... the Jurkat cells were transduced with TCR lentivirus at an estimated multiplicity of infection (MOI) of 5 with 1 μl of polybrene at a final concentration of 4 μg/ml (Sigma, St. Louis, MO). Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was carried out at 4°C overnight with the following antibodies: mouse anti-Calbindin-D-28K (Sigma, C9848, 1:1000), rabbit anti-vGlut2 (Synaptic Systems ...
-
bioRxiv - Microbiology 2023Quote: ... 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
-
bioRxiv - Plant Biology 2023Quote: ... The endocarp was submerged in 1:4:5 v/v/v mixture of 30% hydrogen peroxide (HX0635-2; EMD Millipore, Burlington, MA, USA), distilled water ...
-
bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were infected with the corresponding lentiviruses (1/5 – lentivirus/cell culture medium) in the presence of 4 μg/mL polybrene (Sigma-Aldrich, cat# 107689). 4 days post-infection ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissues in fixed samples were blocked for 4 hours and then incubated using primary antibodies overnight: αH3P 1:250 (Millipore Cat# 05-817R); 6G10 1:500 (DSHB Cat# 6G10-2C7) ...
-
bioRxiv - Cell Biology 2023Quote: Cells were arrested in prometaphase by incubating them for 2 – 4 h in 200 ng ml-1 nocodazole (Sigma-Aldrich, 31430-18-9). Mitotic exit was induced by adding flavopiridol (Tocris Bioscience ...
-
bioRxiv - Neuroscience 2023Quote: ... To identify cholinergic neurons tissue was incubated in rabbit anti-choline acetyltransferase (ChAT, the synthetic enzyme for acetylcholine) for three days at 4°C (1:150; Cat#AB143, EMD Millipore, Billerica, MA) followed by incubation in a secondary antibody coupled to a green (donkey anti-rabbit AF488 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then treated with primary antibody overnight at 4° C with the following antibodies: neuronal marker anti-NeuN at 1:200 (EMD- Millipore, Temecula, California; AB_2298772); astrocyte marker anti-GFAP at 1:400 (Dako ...
-
bioRxiv - Physiology 2023Quote: ... bats were fed with mango nectar (> 95% of the composition in the form of simple sugars - Serigy®) supplemented with 4 mg L−1 of hydrolyzed casein (Sigma Aldrich®). We used the approach adopted by Bozinovic et al ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Microbiology 2022Quote: ... HB10Y samples were titered prior to freezing by serially diluting samples in 10 millimolar (mM) of 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES, Sigma PN#H4034-100G) + 10% Sucrose (Sigma PN #S7903-250G) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: Animals were administered Euthanasia-III Solution (sodium pentobarbital) and transcardially perfused with 1% potassium phosphate buffer solution and 4% paraformaldehyde (Sigma-Aldrich; MO, USA). The seminal vesicles of male subjects and the uterine horns of female subjects were removed and weighed ...
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 1h in TBST containing 4% dried milk and rabbit anti-MYO10 (1:300, Sigma-Aldrich Ref. HPA024223) as primary antibodies ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
-
bioRxiv - Genetics 2023Quote: Fish were fixed overnight in 4% PFA and washed with PBS (3x) and PBS with 0.1% Triton X-100 (Sigma, cat.no.9002-93-1). Larvae were kept overnight in 30% sucrose (Merck ...
-
bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were incubated overnight at 4°C with primary antibody in blocking solution : anti-vinculin (Sigma-Aldrich, catalogue no. V9131, dilution 1:400) followed by three washes with PBS Tween 0.2% ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 1h in a blocking solution of 0.01 M PBS with 0.25% Triton-X and 3% normal goat serum (NGS) and then incubated overnight at 4 °C with rabbit anti-NeuN antibody (1:2000, MAB5504, Millipore, Temecula, CA, USA) in 0.01 M PBS with 0.25% Triton-X and 3% NGS ...
-
bioRxiv - Neuroscience 2024Quote: ... Labeling was performed overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse IgG2a anti- CASPR 1:250 (Millipore Sigma, cat# MABN69), chicken anti-MBP 1:200 (Aves Labs ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the following primary antibodies diluted in 5% TBST: GluA1-C (Millipore, Cat# AB1504, 1:1000), GluA2-C (NeuroMab ...