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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 5’-RACE samples were prepared with 20 μg of RNA being treated with Tobacco Acid Pyrophosphatase (Millipore/Sigma) for 1 hour at 37°C to remove 5’ triphosphates ...
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bioRxiv - Microbiology 2022Quote: ... 5’-RACE samples were prepared with 20 μg of RNA being treated with Tobacco Acid Pyrophosphatase (Millipore/Sigma) for 1 hour at 37°C to remove 5’ triphosphates ...
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bioRxiv - Biochemistry 2023Quote: ... The GCL reaction was stopped by precipitation of proteins with 200 mM 5-sulfosalicylic acid (Sigma Aldrich, 247006) and then put onto ice for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Three different replicas of the irradiated cell lines and the negative control were incubated at 37°C in a 5% CO2 atmosphere for 48 h with the thymine analog 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich, St. Louis, MI, USA). Colcemid was added 24 h before the extraction ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
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bioRxiv - Cell Biology 2020Quote: ... washed and developed with chromogenic 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma-Aldrich, B6149).
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bioRxiv - Physiology 2020Quote: ... and T3 (3,3’,5-triiodo-L-thyronine, > 95% HPCL, CAS number 6893-02-3, Sigma-Aldrich) were first dissolved in 0.1M NaOH and then diluted in 0.9% NaCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-5 μL of biliverdin hydrochloride (chromophore for iRFP signal activation, final concentration 0.002%, Sigma; 30891) added to cells and incubated for 10 mins at 37 °C before imaging ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Sigma Argentina).
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bioRxiv - Neuroscience 2020Quote: ... Then washed for 5 min x 3 times and blocked in 1% BSA (A3059, Sigma-Aldrich) (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were washed 3×5 minutes in TBST buffer and incubated with ChAT antibody (Sigma, AB144) at 1:100 dilutions overnight at 4°C in the blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were allowed to differentiate for 5 days with differentiating media [3 μg/ml prolactin (Sigma, #L6520 ...
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bioRxiv - Cell Biology 2022Quote: ... The reagents treated for some indicated experiments include: 3-5 µM oligomycin A (Sigma, Cat# 75351), 1 µM (for HeLa cells ...
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bioRxiv - Neuroscience 2021Quote: ... 300 μM of the PKA activator 8-Bromoadenosine 3’,5’-cyclic monophosphate (Sigma, dissolved in water) and without FBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS 3 times and blocked with 5% normal donkey serum (Sigma-Aldrich) in wash buffer for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... and guanosine-5’-γ-3-thiotriphosphate (GTPγS) were acquired from Sigma-Aldrich (St. Louis, MO, USA). WIN 55,212-2 was purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were infected with pLKO.1 control or pLKO.1-shZSCAN4 (5’-GAATGCAACAACTCTTGTAATCTCGAGATTACAAGAGTTGTTGCATTCT-3’, Millipore Sigma) and further selected with Puromycin (1ug/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich] ...
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bioRxiv - Bioengineering 2023Quote: ... All reaction mixtures contained 250 nM of sirtuin (SIRT1-3, 5 Reaction Biology, SIRT4,6,7 Sigma-Aldrich), 10 µM furimazine (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were maintained at <3% parasitemia and routinely synchronized by treating with 5% sorbitol solution (Sigma) for ring stage and by using percoll gradient (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Systems Biology 2024Quote: ... 8-Br cAMP (8-Bromoadenosine 3′,5′-cyclic monophosphate, final conc. 500 µM; Sigma B6386-100mg). In addition to stimulation for decidualization ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed (3 times, 5 min each) with PBS containing 0.1% Triton X100 (Sigma-Aldrich), and then incubated with secondary antibody (Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Physiology 2024Quote: ... tissues were washed (3 x 5 min) in PBS containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated with mouse anti-TARDBP primary antibody or mouse anti-HuC/D (for total neurons ...
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bioRxiv - Bioengineering 2024Quote: ... KG) followed by incubation in freshly made 5% (3-Aminopropyl) triethoxysilane (Sigma-Aldrich, Cat. no. 440140) in deionized water for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’) (NC, Sigma, St. Louis, MO, USA). A total of 60 nM siRNAs was used for transfection using Lipofectamine-RNAi-MAX Reagent (13778 ...
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bioRxiv - Developmental Biology 2024Quote: ... Staining was detected with 5-bromo-4-chloro-3’-indolylphosphate/nitro-blue-tetrazolium (BCIP/NBT, Sigma).
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bioRxiv - Microbiology 2020Quote: Biofilms were set up as described previously except they were incubated at 37°C in either 0.03% CO2 or 5% CO2 for 48h in RPMI-1640 containing varying concentrations of the Fe2+ chelator 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p’-disulfonic acid monosodium salt hydrate (Ferrozine – Sigma-Aldrich, 160601) or the Fe3+ chelator Deferasirox (Cambridge Bioscience ...
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bioRxiv - Physiology 2021Quote: ... 100 µl homogenate were added to 400 µl of ice-cold methanol/water 4:1 (v/v) / 0.2% formic acid and isotope-labeled internal standards (10 µl 2 µM 13C3,15N-3-hydroxykynurenine (Sigma-Aldrich) and 10 µl 2 µM D5-kynurenic acid (Eurisotop)) ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Neuroscience 2022Quote: ... 50μM 2-Bromohexadecanoic acid (2-BP) (Sigma-Aldrich, Cat# 238422), 100μM Cycloheximide (CHX ...
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bioRxiv - Cell Biology 2020Quote: ... Unreacted dye was quenched with the addition of 5 mM 2-Mercaptoethanol (Sigma-Aldrich). Free dye was removed with 3 sequential 2 ml Zeba desalting columns (ThermoScientific ...
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bioRxiv - Cell Biology 2020Quote: ... or vehicle (PEG400 / Ethanol / Kolliphor® EL / water, 10:5:2:83, Sigma-Aldrich) in their drinking water ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-tubulin (Sigma, Cat no. T5618, Clone B-5-1-2; 1:200), anti-Muc1 (Cell Signaling Technologies ...
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bioRxiv - Immunology 2021Quote: ... or a monoclonal mouse anti-tubulin antibody (Sigma-Aldrich, Clone B-5-1-2). Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2020Quote: ... The cell aggregates were transferred to 5% Poly(2-hydroxyethyl methacrylate) (Sigma-Aldrich, P3932) coated tissue culture plates at day 7 and cultured in RPMI with B27 supplement until the end of differentiation ...
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bioRxiv - Molecular Biology 2021Quote: ... for 3.5 minutes at 5 μL/min with 2% ACN (Sigma Aldrich, Cat# 1207802), 0.1% formic acid (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were first treated with 10 mM 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) pulses at designated stage and then fixed with fresh 4 % paraformaldehyde (PFA ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Cancer Biology 2020Quote: SNU387 cells were treated with 1.25 uM of 5-aza-2’-deoxycytidine (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: Mice received three intraperitoneal injections (100mg/kg) of 5’-bromo-2 deoxyuridine (BrdU, Sigma) at 4 h intervals and were sacrificed after 28 days ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 5 min with 4,6-diamidino-2-phenylindole (DAPI; Sigma, D9542), washed again three times in PBS and then mounted in Vectashield (Vector Labs) ...