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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
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bioRxiv - Plant Biology 2020Quote: ... and 10 μCi [γ32P]-ATP in reaction buffer (25 mM Tris-HCl, pH 7.5, 5 mM MgCl2, 0.5 mM CaCl2, 2 μM CaM [Sigma-Aldrich], 1 mM DTT) at 30°C for 30 min to perform the vitro kinase assay ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM 2-mercaptoethanol) and concentrated up to 1 nmol/ml (1A260 = 69 pmol) using Amicon Ultra centrifugal filters (Millipore, MWCO 100,000). Further ...
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bioRxiv - Biophysics 2020Quote: ... The DNA construct is attached to the streptavidin coated beads by incubating 0.5 μl of picomolar DNA stock solution and 2 μl beads in 250 μl 1x phosphate buffered saline (PBS, Sigma-Aldrich, USA) for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... and 500 µL of each lysate sample was incubated for 2 h at room temperature with 10 µL of 5 mM biotin picolyl azide (in DMSO) (Sigma Aldrich 900912), 10 µL of 50 mM TCEP (in water) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Microbiology 2021Quote: The pieces of residue (each weighing approximately 5 g) were soaked in 2 mL of phosphate-buffered saline (PBS, Sigma-Aldrich P4417) supplemented with Tween 20 (0.05% v/v ...
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bioRxiv - Neuroscience 2021Quote: ... animals from all groups were injected intraperitoneally with the thymidine analogous 5-bromo-2’-deoxyuridine or bromodeoxyuridine (BrdU, 50 mg/kg; Sigma-Aldrich, US) that is incorporated in the DNA during the S-phase ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μl 1x PBS and 2 μl (1 U) PNGase F (from Elizabethkingia meningoseptica, expressed in E. coli, Sigma Aldrich F8435-50UN) were added to 40 μl of SEC purified YFP-S-RBD (0.24 mg/ml protein concentration) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Immunology 2021Quote: ... and homogenized with a 2 ml dounce grinder for 5 strokes with A motor and at least 20 strokes with B motor (Sigma-Aldrich D8938). The lysates were filtered through 100 µm and 50 µm cell strainers (CellTrics filters 04-004-2328 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using blasticidin (2 µg/ml Sigma-Aldrich, Poland). Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3 ...
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bioRxiv - Plant Biology 2021Quote: ... 10 mM MgCl2, 250 mM sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1X protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]), and carefully overlaid on top of 500 μL of nuclear resuspension buffer 3 (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... for 36-48 h were plated on the SNS imaging plate along with 2 or 5 unit/mL of PI-PLC (P8804, Sigma Aldrich, USA) in complete growth medium for 1 h and then stained with phalloidin-Alexa 405 prior to imaging ...
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bioRxiv - Cell Biology 2021Quote: ... 1 volume of AluI-stock was added to 2 volumes of 5 mg ml−1 fluorescein (FITC) labelled 500 kDa dextran fraction (Sigma-Aldrich, FD500S) dissolved in injection buffer (50 mM K-HEPES ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Immunology 2022Quote: ... we used a synthetic cathepsin C inhibitor (IcatCXPZ-01; (37) diluted in 10 % (2-Hydroxypropyl)-β-cyclodextrin (CAS: 128446-35-5; C0926; Sigma-Aldrich) suspended in citrate buffer 50 mM at pH=5 (vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
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bioRxiv - Neuroscience 2022Quote: Snap frozen retinal samples from 5 older and 5 young primates from both central macular and peripheral regions were homogenized in 2% sodium dodecyl sulfate (SDS, Sigma Aldrich, UK) with protease inhibitor cocktail (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Physiology 2023Quote: Mice were lightly and briefly (< 5 min) anesthetized with 2% isoflurane and injected with 40% w/v PEG-8000 (Sigma-Aldrich, 89510) in sterile 0.9% NaCl subcutaneously using a 28G insulin syringe ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μg of each protein sample was reduced with 5 mM tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich, Cat No: C4706) and then diluted in 50 mM Tris HCl ...
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bioRxiv - Physiology 2023Quote: ... glycerol:relaxing solution (in mM: 100 KCl, 10 imidazole, 2 EGTA, 5 MgCl2, and 4 ATP) containing 1X protease inhibitor cocktail (Sigma-Aldrich P8340) and 1% triton X-100 ...
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bioRxiv - Pathology 2023Quote: ... the samples were rinsed (1 × 10 sec) and washed with PBS (2 × 5 min) before mounting with glycerol (G5516, Sigma Life Science) and covering with #1.5 coverslips of 22 mm × 22 mm (631-0125 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then cultured in fresh RPMI medium containing 30 μM 5-chloro-2′-deoxyuridine (CIdU) (Sigma-Aldrich, St. Louis, MO) and incubated for an additional 4h ...
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bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
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bioRxiv - Neuroscience 2023Quote: ... For each lane we treated 15-20 μg protein samples with 4X NuPAGE LDS Sample Loading buffer (Thermo #NP0007) and 5% 2-mercaptoethanol (Sigma #M3148-25ml), heated them at 70°C for 6 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Molecular Biology 2023Quote: HEK 293T and HeLa cells were treated with 500 μM 5-PT and stimulated with 2 μM calcium ionophore (Sigma-Aldrich, A23187) during incubation ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were incubated at 37 °C in a humidified oven for 2 hours with MTT (Sigma‒ Aldrich, 5 µg/ml). The medium was removed ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... timed pregnant Foxp1flox/flox dams were intraperitoneally injected at specified embryonic days (E12-E17) with a 50 mg/kg dose of 5- Bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich B5002) per gram of body weight of a 20 mg/mL BrdU solution dissolved in PBS or with EdU (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were first permeabilized with 0.1% Triton X-100 in PBS (PBST) for 5 mins and then incubated for 2 hours with Atto 565 Phalloidin (Sigma-Aldrich # 94072) diluted 1:100 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G). Transduced cells were selected by either puromycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... three separate groups of rats (n = 6 to 7 per group) had received 5-bromo-2’-deoxyuridine (BrdU; Sigma, St. Louise, MO) in phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM ammonium acetate pH 5 (Sigma-Aldrich 73594), 50% methanol (Honeywell LC230-4) ...
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bioRxiv - Genetics 2020Quote: For survival to 5-azacytidine (5-azaC, Sigma Aldrich), MG1655 and isogenic derivative (Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 nmol/L 3,3’,5-triiodo-L-thyronine (Sigma), 1 μmol/L dexamethasone (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... 5-(iodoacetamido)fluorescein (5-IAF) was from Sigma-Aldrich. SAM was purchased from New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μM S-(5’-Adenosyl)-L-methionine (Sigma A7007), 20 U SUPERase·In (Invitrogen AM2694) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol with addition of 5 mM desthiobiotin (Sigma).
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bioRxiv - Plant Biology 2024Quote: ... 1 mg/mL 5-FOA (5-Fluoroorotic acid, Sigma) was added to the selection plates ...
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...