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Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... trabectedin was thawed and formulated in 0.1 g of Hydroxy B-Cyclodextrin (Sigma-Aldrich, St. Louis, MO, MKBL6868V) and sterile PBS at 0.0533 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Neuroscience 2023Quote: Mice were fasted for 2-5 hours prior to oral gavage of 4-kDa (Sigma-Aldrich, 600 mg/kg body weight, 80 mg/mL PBS) by gavage ...
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bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Microbiology 2024Quote: ... Cells were cells fixed with 4% paraformaldehyde (ProSciTech, Cat. no. C004) in PBS for 30 min at RT and blocked with 2% BSA (Sigma-Aldrich, Cat. no. A8022-10G) in PBS (BSA/PBA ...
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bioRxiv - Immunology 2024Quote: On GD10, sixty-five females were anesthetized with 2% inhaled Isoflurane (BoChimico, Itatiaia, RJ, Brazil) and perfused with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) in PBS (50mM) ...
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bioRxiv - Cell Biology 2020Quote: ... complete medium containing 20 mM 2-deoxyglucose (2-DG; Carl Roth GmbH, Karlsruhe, Germany) and 10 M antimycin-A (Sigma-Aldrich, Steinheim, Germany) for at least 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the affinity gel was suspended in elution buffer (2% SDS, 50 mM Tris pH 7.5, 50 mM NaCl, 20% glycerol, 2 mM dithiothreitol (Sigma-Aldrich #D0632-5G; fresh) and incubated at 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: The mouse was induced by injecting 2 month-old female BALB/c mice with2 mg human-derived proteoglycan (PG) together with 2 mg DDA (Sigma, MO, united States). as described previously (1) ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...
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bioRxiv - Microbiology 2023Quote: hPCLS were infected with SARS-CoV-2 at 2×105 TCID50 per slice and treated with homoharringtonine (HHT) (Sigma, cat# SML-1091-10MG) at 1 h p.i ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2; Catalog no. H7041; Sigma, St. Louis, MO). All the cells were incubated at 37°C in the presence of 5% CO2.
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bioRxiv - Biophysics 2023Quote: Agarose gels were prepared at 2% (weight) in Tris-Borate-EDTA (TBE, Sigma-Aldrich, 89 mM Tris-borate, 2 mM EDTA, pH 8.3) buffer ...
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bioRxiv - Genomics 2023Quote: ... the following modifications were made to the initial extraction: 2% (w/v) polyvinylpyrrolidone (PVP, average molecular weight 40,000) and 10% 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) were added to the lysis buffer ...
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bioRxiv - Physiology 2024Quote: ... 8.0x104 cells/well were seeded the night prior on a 96-well plate and treated with 5mM 2-deoxy-D-glucose 2h prior to the initial seahorse reading (2-DG; Millipore Sigma cat. D2134). ATP production rates from glycolysis (glycoATP ...
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bioRxiv - Developmental Biology 2024Quote: ... The following chemical compounds were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375); oligomycin-A (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... in 2× SSC buffer supplemented with 2 mM of the unspecific RNase inhibitor vanadyl ribonucleoside complex (VRC, Sigma-Aldrich, St. Louis, MS, USA) for 15 min at RT ...
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bioRxiv - Biophysics 2024Quote: Saturated salt solutions were prepared by adding Mg(NO3)2·6 H2O and Co(NO3)2·6 H2O (both from Sigma-Aldrich Sweden AB) to H2O (Milli-Q quality ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatants containing lentiviruses were mixed 1:1 with target cell media and supplemented with 4 mg/ml polybrene (Sigma-Aldrich cat #107689). Successfully transduced cells were selected using puromycin (Cedarlane cat #13884-50) ...
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bioRxiv - Bioengineering 2020Quote: The following primary antibodies were incubated for 1 hour at room temperature or for 12-18 hours at 4°C for the following markers: rabbit PCM-1 (1:100 dilution, Sigma-Aldrich, HPA023370-100UL), mouse PCM-1 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were refed with fresh media containing puromycin (4 μg/ml) and doxycycline (Sigma, D9891; stock 1 mg/ml, diluted 1:1000), which was also added to the cells daily thereafter ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (anti-lamin B1, ab16048, Abcam, 1:5000; anti-β-Actin, A1978, Sigma, 1:10,000) in 4% BSA/TBS + 0.025% sodium azide ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.3% Triton-X 100 in PBS for 1 hr before a 48-hr incubation at 4°C in 1:1,000 mouse anti-TH (Millipore; St. Louis, MO, USA) and 1:3,000 rabbit anti-cFos (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer at the following dilutions and incubated on tissue sections at 4°C overnight: Calb1 (Swant, CB-38a, 1:1000) and Pvalb (Millipore-Sigma, MAB1572, 1:1000). Sections were then washed three times PBS and incubated in anti-mouse or anti-rabbit fluorescently conjugated secondary antibodies (Invitrogen Life Technologies ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... membranes were incubated overnight on gentle agitation at 4°C with primary antibodies (TH, 1:1000; GAPDH, Sigma, G8795, mouse monoclonal, 1:10,000) diluted in antibody buffer (1:1 of Tris buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were mixed with an equal volume of 1× PBS solution containing 1 mM potassium 4-methylumbelliferyl sulfate (SIGMA ALDRICH, Castle Hill, Australia) and 2 mM phenol (SIGMA ALDRICH ...
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bioRxiv - Neuroscience 2023Quote: ... overnight and then with primary antibodies for 48 h at 4°C: rabbit-α-rab3 (1:250; provided by S.J. Sigrist, unpublished) and chicken-α-GFP (1:150, Sigma Aldrich, AB_90890). After a further round of washing ...
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bioRxiv - Molecular Biology 2022Quote: ... cerevisiae was spiked with S. pombe total RNA (4:1 ratio S. cereviasiae : S. pombe)treated with 4-tU 500 μM final concentration (Sigma-Aldrich, 440736). The biotinylation was performed with 200ul EZ-link-HPDP-biotin (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... were dissected as mentioned above and fixed with 2% paraformaldehyde and 0.06% glutaraldehyde in PEM (0.1 M PIPES: P1851-500g, Sigma, Germany; 2 mM EGTA: E3889, Sigma, Germany; 1 mM MgSO4 x 7 H2O, v/v) for 90 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... HD cells were washed with PBSAG (phosphate buffer saline [PBS, Sigma-Aldrich] + 1 mg/ml bovine serum albumin [BSA, Sigma-Aldrich] + 2 mg/ml glucose [Sigma-Aldrich]) and stored at 4°C for a maximum of 1 week.
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) at 4 °C for 10 min before 3% of Ficoll-400 (Sigma) was added and assembled Nucleolin complexes were resolved on a 10% TBE PAGE gel at 4 °C.
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bioRxiv - Molecular Biology 2021Quote: ... for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma). Samples and results were analyzed by FACS-Aria and FlowJo (FlowJo ...
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bioRxiv - Developmental Biology 2021Quote: ... Specimens were counterstained with DAPI overnight at 4°C (Sigma, 1 μg/ml in PBSTx).
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bioRxiv - Evolutionary Biology 2022Quote: ... consisting of 4% (v/v) of 19:1 acrylamide/bis-acrylamide (Sigma-Aldrich, A9926-5), 60 mM Tris·HCl pH 8 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with mEM48 (1:500, Merck Millipore, Cat. # MAB5374) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed with PBS plus 1% BSA and then fixed in 4% paraformaldehyde (Sigma) followed by processing for EdU conjugation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in HRP-anti-DIG antibody (1/1500, Sigma) in TNB ...
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bioRxiv - Biochemistry 2020Quote: ... 0.625 mM TBTA (Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine) (Merck Millipore), and 6.25 mM CuSO4 (Merck Millipore) ...
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bioRxiv - Pathology 2021Quote: ... tissues were stained overnight at 4°C with rat anti-laminin (1:1000, Sigma L0663) in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... one cage of 4 mice received water with 1 mg/ml doxycycline hyclate (Sigma-Aldrich) at day 4 post infection ...
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bioRxiv - Cancer Biology 2021Quote: ... LacZ shRNA (control) and 4 different DARPP-32 shRNAs cloned in pLKO.1 plasmids (Sigma) along with their corresponding packaging plasmids were transfected in human HEK-293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... 400 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (678937, Sigma, UK) and 8 mM ascorbic acid (A15613 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C for 5min and one wash with a PBS containing 1% BSA (Sigma-Aldrich). The single-cell suspensions used for 10X Genomics scRNA-seq analysis were cleared of red blood cells by using a red blood cell lysis bufer (eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore). Beads were washed with GTEN buffer ...
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bioRxiv - Microbiology 2021Quote: ... Chromatin was fragmented by incubating the cells with 4 U µl−1 MNase (Sigma-Aldrich) for 10 min at 25°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were fixed 1 week after passaging with 10% formalin solution containing 4% paraformaldehyde (Sigma) for 15 min at RT ...