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1 - 10 of 10 citations for 6 AMINO 2 5 DINITROPYRIDINE since 2019

• Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2)

  Apirat Chaikuad, et al., bioRxiv - Biochemistry 2019

  Quote: ... the 5 uL kinase reaction was performed with 2 ug/mL recombinant human ULK1 protein (1-649, SignalChem #U01-11G) and 80 ug/mL myelin basic protein (MBP ...
• Novel substrate prediction for the TAM family of RTKs using phosphoproteomics and structure-based modeling

  Naomi E. Widstrom, et al., bioRxiv - Biochemistry 2023

  Quote: ... 2 mM ATP) and 2 ug of recombinant kinase (SignalChem; Tyro3 (aa 455-end): T22-11G ...
• Calcium-dependent regulation of neuronal excitability is rescued in Fragile X Syndrome by a tat-conjugated N-terminal fragment of FMRP

  Xiaoqin Zhan, et al., bioRxiv - Neuroscience 2024

  Quote: ... 0.1 μg/ml MEK1 activated extracellular signal-regulated kinase 1 and 2 (pERK1/2) (SignalChem Biotech), 1 μM TTA-P2 (Alomone Labs) ...
• Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment

  Gabriela Traczyk, et al., bioRxiv - Cell Biology 2023

  Quote: ... 2 - 30 ng of GST-hDGKε (SignalChem, cat. No. D24–10G) was applied onto the gels ...
• The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment

  Xiaoshan Shi, et al., bioRxiv - Biochemistry 2020

  Quote: 100 nM purified ULK1 complex was mixed with 5 µM ULKtide (SignalChem Biotech Inc.), and incubated at room temperature for 1 h ...
• Allele-specific activation, enzyme kinetics, and inhibitor sensitivities of EGFR exon 19 deletion mutations in lung cancer

  Benjamin P. Brown, et al., bioRxiv - Cancer Biology 2022

  Quote: ... L747_A750>P (#E10-12MG, lot G1200-3), and L747_E749 (#E10-12LG, lot G1344-5) were purchased from SignalChem. The Promega ADP-Glo™ kinase assay kit was used to quantify the amount of ADP produced by each EGFR variant in 1XBFA buffer and in the presence or absence of erlotinib at varying concentrations ...
• Activation and inhibition of the C-terminal kinase domain of p90 ribosomal S6 kinases

  Marlene Uglebjerg Fruergaard, et al., bioRxiv - Biochemistry 2022

  Quote: ... RSK CTKD proteins (2 µM) were either untreated or pre-activated by ERK2 (0.1 µM) (Signalchem) in reaction buffer containing 200 µM ATP and 10 mM MgCl2 in kinase buffer (50 mM HEPES pH 7.0 ...
• Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2)

  Apirat Chaikuad, et al., bioRxiv - Biochemistry 2019

  Quote: ... the 5 uL reaction was performed with 4 ug/mL recombinant human ULK2 protein (1-478, SignalChem #U02-11G) and 80 ug/mL MBP in the presence of 25 uM ATP ...
• The FDA-approved drug Alectinib compromises SARS-CoV-2 nucleocapsid phosphorylation and inhibits viral infection in vitro

  Tomer M. Yaron, et al., bioRxiv - Microbiology 2020

  Quote: ... All the recombinant kinases (SRPK1/2/3, GSK-3α/β and CK1A/E were obtained from SignalChem.
• TBK1 interacts with tau and enhances neurodegeneration in tauopathy

  Measho H. Abreha, et al., bioRxiv - Neuroscience 2020

  Quote: ... 2 mM EDTA and 0.25 mM DTT) plus 100 μM ATP for 30 min at 37°C (SignalChem, K01-09). TBK1 kinase activity was inhibited by adding 100 μM or 200 μM of BX795 (ENZ-CHM189-0005 ...