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Citations for GENEWIZ :
1 - 36 of 36 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... pCAGGS-SARS-CoV-2-S that encodes the SARS-CoV-2 Spike gene was generated by GENEWIZ (Suzhou, China). Recombinant adenovirus expressing human ACE2 (Ad5-hACE2 ...
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bioRxiv - Microbiology 2021Quote: ... RBD fragment (aa319-541) of SARS-CoV-2 S protein (GenBank: MN908947.3) was synthesized by Genewiz Inc (GENEWIZ, Suzhou, China), the extracellular domain of human ACE2 (1-740 aa ...
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bioRxiv - Microbiology 2022Quote: ... The beta variant of the SARS-CoV-2 spike protein (L18F, D80A, D215G, del242/243, R246I, K417N, E484K, N501Y, A701V) was synthesized in two fragments (GENEWIZ, South Plainfield, NJ, USA) and cloned into the pcDNA3.1+ expression vector (Cat# V79020 ...
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bioRxiv - Cell Biology 2022Quote: ... S403D (S -> D and T -> E to mimic the size of the aa)) was designed in silico and synthesised by GENEWIZ Co ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid substitutions and deletions in the SARS-CoV-2 spike protein (shown in Figure 2) were introduced using standard molecular biology techniques135 and confirmed by Sanger sequencing (GENEWIZ). When indicated ...
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bioRxiv - Microbiology 2022Quote: ... library preparation and Hi-Seq 2 x150 paired-end sequencing were performed by GENEWIZ (New Jersey, USA).
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bioRxiv - Biochemistry 2021Quote: ... coli expression by GENEWIZ. Expressed K-RAS proteins were full-length with the C-terminal two amino acids removed to mimic post-translational processing (Hancock et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and Cc07_g06570 (Table S2) were synthesised de novo by GENEWIZ (Suzhou, China) as Golden Gate MoClo/Phytobrick B3–B4 CDS parts (Patron et al. ...
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bioRxiv - Cell Biology 2022Quote: ... coli expression and synthesized (GENEWIZ). The sequence of VASH2 was modified by mutating Cys158 to alanine resulting in a catalytically inactive protein (Wang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... nsp8 (residues 1-198) and nsp12 (residues 1-932) (GenBank accession number MN908947) were all chemically synthesized with codon optimization (S2 Table) (GENEWIZ). The −1 ribosomal frameshifting that naturally occurs in virus to produce nsp12 was corrected in the synthesized gene to express nsp12 protein ...
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bioRxiv - Microbiology 2022Quote: Heavy and light chain amino acid sequences were downloaded from the CoV-AbDab database and synthesized as codon-optimized gBlock fragments (GENEWIZ). These antibodies were expressed using the Expi293 expression system kit (Cat# A14635 ...
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bioRxiv - Neuroscience 2021Quote: ... and obtained the human FLAG-APLP1(mut9) gene with a Flag tag in the N-terminal synthesized by GENEWIZ Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... coli and plasmid was extracted using Qiagen Miniprep columns and clones were confirmed by Sanger Sequencing (GENEWIZ). Whole plasmid sequence was confirmed by nanpore long read sequencing (Plasmidsaurus) ...
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bioRxiv - Microbiology 2022Quote: ... We generated standard curves using a 10-fold serial dilution of the standards by cloning the 18S region of the fungus Saccharomyces cerevisiae or the 16S region of the bacteria Escherichia coli into puc57 plasmid vectors constructed by GENEWIZ, Inc ...
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bioRxiv - Genetics 2021Quote: ... mek-2 and gpdh-2 was synthesized and cloned into the L4440 vector by GENEWIZ (Takeley, UK). Vectors were transformed in E ...
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bioRxiv - Microbiology 2019Quote: ... Each SAG library was sequenced using an Illumina MiSeq 2 × 75bp or HiSeq 2 × 150bp configuration (GENEWIZ).
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bioRxiv - Cancer Biology 2022Quote: ... and Dox 4 days (Day 4) thyroid (n=4; 2 males and 2 females for each group) were sequenced by GENEWIZ-NGS Europe (Germany ...
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bioRxiv - Systems Biology 2021Quote: ... and sequenced on Illumina HiSeq 2 × 150 bp paired-end sequencing (GENEWIZ).
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bioRxiv - Molecular Biology 2022Quote: ... ∼450M read pairs with 2×150 bp sequencing by GENEWIZ (Leipzig, Germany).
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bioRxiv - Microbiology 2023Quote: ... Purified genomic DNA was paired end sequenced (2 x 150 bp) (GENEWIZ). Sequence libraries were prepared with the NEBNext Ultra II FS DNA Library Prep 136 kit (New England Biolabs GmbH ...
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bioRxiv - Microbiology 2020Quote: ... library preparations and HiSeq 2×150 Paired End sequencing were performed by GENEWIZ (South Plainfield, NJ, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Targeted amplicon library preparation and NGS sequencing (MiSeq; 2 x 250bp) were performed at Azenta (previously GENEWIZ, Inc.) and analyzed using CRISPResso2 ...
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bioRxiv - Immunology 2021Quote: ... The coding sequence for SARS-CoV-2 RBD spanning S protein 319-541 (GenBank: YP_009724390) was codon-optimized for mammalian cells and synthesized by GENEWIZ, China ...
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bioRxiv - Biophysics 2022Quote: ... Paired-end sequencing (2×150 bp) was then performed on an Illumina NovaSeq next generation sequencer for HeLa cells (GENEWIZ) and (2×75 bp ...
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bioRxiv - Genomics 2020Quote: ... The mRNA libraries were created using TruSeq RNA kit (LANGEBIO, CORE-FACILITY) and sequenced on an Illumina HiSeq 2500 platform using 2×150 paired-end format (GENEWIZ). The small RNA libraries were created using the Illumina TruSeq small RNA kit and sequenced on an Illumina HiSeq 2500 with a 1×50 single-end format (GENEWIZ).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cell lines as described elsewhere (42) and sequenced on an Illumina HiSeq instrument (2×150 paired-end, 100 million reads/sample) by GENEWIZ, LLC ...
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bioRxiv - Cancer Biology 2021Quote: ... Strand-specific poly-A selected RNA libraries were sequenced on Illumina HiSeq platform with 2×150 bp read length to an average of 200M reads per sample by GENEWIZ. All raw genomic data is available upon access request through the Children’s Brain Tumor Network (https://cbtn.org/ ...
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bioRxiv - Neuroscience 2022Quote: RNA-seq files were provided in the format of 2×150 bp paired-end (∼25 M reads per sample) fastq files from GENEWIZ. Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... RNA-seq files were provided in the format of 2×150 bp paired-end (∼25 M reads per sample) fastq files from GENEWIZ. Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing was performed using Illumina HiSeq system in a 2 x 150-bp configuration (single index, per lane) by GENEWIZ. Starting from the raw .fastq files ...
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bioRxiv - Immunology 2022Quote: ... Sequencing was performed on Illumina HiSeq platform (2 × 150 bp configuration, single index, 50 million reads per sample) by GENEWIZ.
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bioRxiv - Biochemistry 2021Quote: Libraries were prepared using Illumina HiSeq platform with ultra-low input configuration and sequenced with 2× 150 bp sequencing configuration to a depth of 350 million reads (GENEWIZ, NJ).
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bioRxiv - Plant Biology 2021Quote: ... and sequenced on an Illumina HiSeq or NovaSeq instrument (San Diego, CA) using a paired end 2 x150 bp read length (GENEWIZ, Plainfield, NJ). The final read numbers and mapping results (for all the transcriptomes ...
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bioRxiv - Microbiology 2020Quote: ... Purified DNA was used for PCR amplification of the variable V3 and V4 regions (Using MetaVx.2™ system developed with GENEWIZ (Plainfield, NJ)of the 16S rRNA gene as was previously described in US patent 9,745611 ...
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bioRxiv - Systems Biology 2022Quote: ... Sequencing library was prepared using a MetaVx™ 16s rDNA Library Preparation kit and ITS-2 Library Preparation kit (GENEWIZ, Inc., South Plainfield, NJ, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: All DNA samples were sequenced to approximately 20x coverage using the Illumina HiSeq X Ten with 2×150 paired-end reads (GENEWIZ, Inc., South Plainfield, NJ, USA). Chimpanzee LCLs were sequenced across two separate batches with two samples re-sequenced across batches as a control (NS03621 r=0.99 ...