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Citations for Addgene :
1 - 35 of 35 citations for pRSETB TagBFP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... or tagBFP (Addgene plasmid #163747) fluorescent markers ...
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bioRxiv - Cell Biology 2024Quote: ... TagBFP-FKBP-PIP5K1C (Addgene #220090) (recruitable PIP5K1C) ...
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bioRxiv - Biochemistry 2024Quote: ... NLS-TagBFP (Addgene plasmid #55265) was used in an equimolar amount as a co-transfection marker which was shown to co-express from the same cell as the other transfected plasmid 94% of the time.13 In the 5 µM Cbl-4xGly-ATTO590 experiments only ...
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bioRxiv - Microbiology 2024Quote: ... The amplified DNA was cloned into the plasmid pRSETb (Addgene) at its multiple cloning site C-terminal of a His-tag sequence and transformed into E ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569-; Fuw-dCas9-TET1-P2A-tagBFP -Addgene #108245- and pLV GG hUBC-dsRED -Addgene #85034- ...
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bioRxiv - Neuroscience 2024Quote: ... and a plasmid for tagBFP in our lab stock (TRE-NLS-tagBFP, which is modified from Addgene plasmid #92202). The NLS-tagBFP sequence was PCR amplified from plasmid pAAV-TRE-NLS-tagBFP ...
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bioRxiv - Molecular Biology 2020Quote: ... The TagBFP coding sequence was derived from Addgene plasmid #67168 ...
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bioRxiv - Synthetic Biology 2022Quote: ... we generated the complement InterTag-BFP by inserting the sequence encoding for SpyTag002-sfCherry11 –TagBFP amplified from pSFFV-SpyTag-sfCherry2(11)-TagBFP (Feng et al. [53] Addgene #117485) into the membrane expression vector pDisplay (Addgene #34842 ...
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bioRxiv - Genomics 2023Quote: ... a Neomycin resistance gene and a negative selection marker expressing tagBFP and was derived from pDONOR-tagBFP-PSM-EGFP (Addgene 100603). Flanking the positive selection cassette ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569-; Fuw-dCas9-TET1-P2A-tagBFP -Addgene #108245- and pLV GG hUBC-dsRED -Addgene #85034-) using calcium phosphate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The Perceval-HR coding sequence was adapted from pRsetB-his7-Perceval (Addgene Plasmid# 20336) by codon optimizing for C ...
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bioRxiv - Biophysics 2022Quote: ... coli DH5α/pRsetB-his7-Perceval was a gift from Gary Yellen’s lab (Addgene plasmid #20336). Plasmid pRsetB-his7-Perceval was isolated and transferred into E ...
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bioRxiv - Cell Biology 2024Quote: ... APMAPAD-KDEL (untagged) was made from TagBFP-KDEL (Addgene 49150) by replacing TagBFP between the ER signal sequence of BiP and KDEL ER retention signal with the ER-luminal portion of APMAP ...
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bioRxiv - Genomics 2024Quote: ... and a transfer vector (Fuw-dCas9-DNMT3A-P2A-tagBFP -Addgene #84569- ...
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bioRxiv - Cell Biology 2023Quote: ... tagBFP-Rab35 (Human Rab35; in lentivirus vector pLVX-M-puro (Addgene 125839)) ...
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bioRxiv - Molecular Biology 2024Quote: ... replacing the URA3 marker from pRS316-pTDH3-Su9-TagBFP (Addgene plasmid # 62383) by HIS3 ...
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bioRxiv - Genetics 2024Quote: ... with the pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blast plasmid (Addgene 187956) and a vector carrying a hyperactive PiggyBac transposase (pBROAD3-hyPBase-IRES-zeocin ...
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bioRxiv - Microbiology 2022Quote: The Ca2+ FRET reporter Twitch-2B was PCR amplified from the plasmid Twitch-2B pRSETB obtained from Addgene [23] using the primers T2B1 and T2B2 (Table 1 ...
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bioRxiv - Genetics 2024Quote: ... The puromycin resistance gene and TagBFP regions were amplified from pAX198 (Addgene no. 173042). For NG-PE7 expression plasmid cloning ...
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bioRxiv - Neuroscience 2020Quote: ... and the fluorescent protein cDNA for tagBFP was cloned from pdCas9::BFP-humanized (Addgene, #44247). A zebra finch FoxP2 cDNA clone provided by Erich Jarvis was subcloned into pLenti6.4 using a gateway reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... The pCAG-dGBP1-TagBFP plasmid was a gift from Connie Cepko [49] (Addgene plasmid #80086). Calreticulin overexpression constructs were generated by PCR amplification off of genomic DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... as follows: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9 (Addgene: 84261)36 ...
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bioRxiv - Cell Biology 2024Quote: H2B-tagBFP was cloned by inserting the H2B cDNA (amplified from pLenti6-H2B-mCherry, Addgene 89766) into the tagBFP-N1 vector (Evrogen ...
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bioRxiv - Genetics 2024Quote: ... and blue fluorescent protein (tagBFP) with a KRAB domain at the C-terminus (Addgene 167981; Figure 1A)14 ...
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bioRxiv - Synthetic Biology 2021Quote: ... while pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1- rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting (Addgene #9213925) was used as template for both hGeminin and hCdt1 tags ...
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bioRxiv - Biophysics 2021Quote: ... Cox4-mKate2 was generated by replacing TagBFP with mKate2 in the mito-BFP vector (a gift from Prof. Gia Voeltz (Addgene plasmid #49151 ...
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bioRxiv - Genetics 2022Quote: ... The KRAB-Split-dCas9 plasmid was generated by inserting the FKBP12F36V domain C-terminally to dCas9 into the plasmid pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9 (Addgene plasmid # 84241 ...
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bioRxiv - Genomics 2023Quote: ... 2.106 SOX2-P2A-tagBFP cells were transfected with 50nM of plasmid expressing either miRFP670 (a gift from Vladislav Verkhusha; Addgene plasmid #79987 ...
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bioRxiv - Genomics 2023Quote: ... The original CRISPRoff plasmid DNA (except the region encoding TagBFP) and mScarletI cDNA from the pmScarlet-i_C1 plasmid (Addgene # 85044) were amplified by polymerase chain reaction (PCR ...
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bioRxiv - Genetics 2024Quote: ... The cell line was generated via PiggyBac-mediated transposition of the TX1072 A3 line with the pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blast plasmid (Addgene 187956)41 ...
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bioRxiv - Molecular Biology 2021Quote: ... The IRES-Puro elements in the above plasmids were replaced with IRES-TagBFP from TRE-KRAB-dCAS9-IRES-BFP (Addgene, 85449) to make them compatible with the MeTAFlow assay ...
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bioRxiv - Cell Biology 2023Quote: ... we first generated PB-TAC-ERN-BFP by Gateway recombination between pENTR-tagBFP and PB-TAC-ERN (a gift from Knut Woltjen, Addgene #80475), after which the rtTA/NeoR expression cassette was deleted by BglII/NheI digestion ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV-GfaABC1D-tagBFP-miR30 plasmid was generated by combining the tagBFP sequence with the miR30 sequence (Horizon, pGIPZ plasmid) using overlapping PCR and subcloned into pAAV-GfaABC1D-eGFP plasmid (Addgene #176861). The pAAV-GfaABC1D-tagBFP-miR30-scramble and pAAV-GfaABC1D-tagBFP-miR30-shAckr3 constructs were subcloned into the pAAV-GfaABC1D-tagBFP-miR30 vector with the following target sequences.
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bioRxiv - Bioengineering 2021Quote: ... 5 uL of serum-free media was added to 200 ng of plasmid DNA (100 ng CAG-nanobody-TagBFP plasmid and 100 ng CAG-dsRed plasmid (Addgene plasmid 11151) (Matsuda and Cepko ...
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bioRxiv - Genetics 2021Quote: ... the puromycin resistance cassettes in the piggybac CRISPRi expression plasmid pSLQ2818 (pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9, Addgene 84241 (Gao et al. 2016)were exchanged for a blasticidin resistance ...