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1 - 14 of 14 citations for pDONR207 since 2020

• Two groups of Arabidopsis receptor kinases preferentially regulate the growth of intraspecies pollen tubes in the female reproductive tract

  Hyun Kyung Lee, Daphne R. Goring, bioRxiv - Plant Biology 2020

  Quote: ... and pDONR207 (Addgene). At-BKN1 was cloned from Hh-0 ecotype and other Brassicaceae RK and BKN cDNAs were cloned from B ...
• SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation

  Jung-Hyun Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... pDONR207 SARS-CoV-2 NSP1 (Addgene, 141255), pDONR223 SARS-CoV-2 NSP2 (Addgene ...
• Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

  Jonathan D. Lee, et al., bioRxiv - Microbiology 2023

  Quote: ... pDONR207 SARS-CoV-2 nsp3 (Addgene # 141257), pDONR223 SARS-CoV-2 NSP6 (Addgene #141260) ...
• SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1α in pulmonary epithelial and endothelial cells

  Magdalena Ambrożek-Latecka, et al., bioRxiv - Immunology 2023

  Quote: ... pDONR207 SARS-CoV-2 M and pDONR207 SARS-CoV-2 ORF3a (all plasmids were gifts from Fritz Roth via Addgene, # 141273 ...
• The differential effect of SARS-COV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage and virus evolution

  Juan José Berlanga, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2, Addgene #164523) plasmids using the primers 5-EX-NSP1 and 3-NB-NSP1 and cloned into pEGFP-N1 digested with EcoRI and NotI enzymes ...
• Discovering host protein interactions specific for SARS-CoV-2 RNA genome

  Roberto Giambruno, et al., bioRxiv - Molecular Biology 2023

  Quote: pDONR207 SARS-CoV-2 NSP1 was a gift from Fritz Roth (Addgene plasmid # 141255 ...
• The differential effect of SARS-COV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage and virus evolution

  Juan José Berlanga, et al., bioRxiv - Molecular Biology 2024

  Quote: ... pDONR207 SARS-CoV-2 nsp1 Delta RC (M1, K164A/H165A, Addgene #164522) and pDONR207 SARS-CoV-2 nsp1 R124A/K125A (M2 ...
• The differential effect of SARS-COV-2 NSP1 on mRNA translation and stability reveals new insights linking ribosome recruitment, codon usage and virus evolution

  Juan José Berlanga, et al., bioRxiv - Molecular Biology 2024

  Quote: ... nsp1 coding sequences were amplified by PCR from pDONR207 SARS-CoV-2 nsp1 (WT, Addgene #141255), pDONR207 SARS-CoV-2 nsp1 Delta RC (M1 ...
• SARS-CoV-2 Nsp1 suppresses host but not viral translation through a bipartite mechanism

  Ming Shi, et al., bioRxiv - Cell Biology 2020

  Quote: ... Nsp1-NT (1-127 aa) and Nsp1-CT (128-180 aa) were amplified from pDONR207 SARS-CoV-2 NSP1 (Addgene) by PCR and then cloned into pDB-His-MBP or BacMam pCMV-Dest plasmid ...
• Targeting Stem-loop 1 of the SARS-CoV-2 5’UTR to suppress viral translation and Nsp1 evasion

  Setu M. Vora, et al., bioRxiv - Molecular Biology 2021

  Quote: ... Nsp1-NT (1-127 aa) and Nsp1-CT (128-180 aa) were amplified from pDONR207 SARS-CoV-2 NSP1 (Addgene) by PCR and then cloned into pDB-His-MBP or BacMam pCMV-Dest plasmid ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: UAS-SARS-CoV-2-ORF3a transgenic flies were generated by PCR-mediated subcloning of the SARS-CoV-2-ORF3a coding sequence (pDONR207-SARS-CoV-2 ORF3a, #141271, Addgene) into pUASt-Attb (EcoRI/XbaI) ...
• Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

  Shuo Yang, et al., bioRxiv - Microbiology 2022

  Quote: pCMV-GFP-SARS-CoV-ORF3a was generated by recombining the SARS-CoV-2-ORF3a coding sequence (pDONR207 SARS-CoV-2 ORF3aA, #141271, Addgene) into pDEST-CMV-N-EGFP (#122842 ...
• ISGylation of the SARS-CoV-2 N protein by HERC5 impedes N oligomerization and thereby viral RNA synthesis

  Junji Zhu, et al., bioRxiv - Microbiology 2024

  Quote: ... pcDNA-V5-NSP3 was generated by amplifying the full-length NSP3 ORF from pDONR207 SARS-CoV-2 NSP3 (Addgene #141257; a gift from Fritz Roth (86)) and by subcloning it into the pcDNA3-C-V5 vector ...
• A protease-activatable luminescent biosensor and reporter cell line for authentic SARS-CoV-2 infection

  Pehuén Pereyra Gerber, et al., bioRxiv - Microbiology 2021

  Quote: ... The coding sequence from amino acid 747E to 1061K was amplified by PCR from the pDONR207 SARS-CoV-2 NSP3 plasmid (Addgene, #141257, a gift from Fritz Roth[32]), including a Kozak sequence and ATG start codon in the forward primer and a TAA stop codon in the reverse primer (PLPcd_Fw ...