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Citations for Addgene :
1 - 18 of 18 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and RPA 2 and 3 were cloned into 11A vectors (Addgene: 48294), followed by assembly of all RPA subunits into one vector by successive ligation independent cloning reactions ...
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bioRxiv - Biophysics 2022Quote: ... and 32C (residues 172–270 of RPA2) domains of human RPA were PCR amplified using p11d-tRPA plasmid (Addgene plasmid102613) (Henricksen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells were transfected using Fugene HD transfection reagent in a 3:1 reagent : DNA ratio with packaging plasmid 2 µg psPAX2 (a gift from Didier Trono, Addgene, #12260), 1 µg murine ecotropic envelope plasmid pEnv(eco)-IRES-puro (Morita et al ...
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bioRxiv - Molecular Biology 2021Quote: ... transfection reagent with 60 ng of LwaCas13a plasmid (Addgene ID 91924), 20 ng of gRNA plasmid (generated by cloning oligos into an LwaCas13a crRNA entry plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... transfection reagent and Notch reporter plasmids (#44211, 47684, 47683; Addgene, Cambridge, MA, USA) were combined in serum free OptiMEM media (Gibco ...
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bioRxiv - Synthetic Biology 2020Quote: ... MultiMate-Rainbow and MultiMate-HITI-2c ACTB reagents will be made available for distribution by Addgene. All other reagents are available from the authors upon reasonable request.
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bioRxiv - Biochemistry 2023Quote: ... Transfection reagents were prepared by combining 6.7 μg psPAX2 (2nd generation lentiviral packaging plasmid, Addgene #12260), 0.85 μg pVSV-G (Expresses VSV-G envelop protein for pseudotyping NanoMEDIC particle ...
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bioRxiv - Neuroscience 2021Quote: ... 3 × 106 HEK293T cells were transfected using GenJet transfection reagent (Signagen) with 7.5 µg psPax2 (Addgene #12260), 5 µg VSV-G (pMD2.G ...
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bioRxiv - Microbiology 2024Quote: The full-length HIV vectors NL4-3 ΔEnv EGFP (HIV Reagent Program) and HIVGKO (Addgene plasmid #112234) were produced in HEK293T cells along with the VSV-G (Addgene plasmid # 8454 ...
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bioRxiv - Immunology 2021Quote: ... an envelope deficient HIV-1 dual reporter construct that was cloned by recombination of the pNL.luc.R-E-plasmid (NIH AIDS Reagent Program) and the fully infectious pNL4-3 mCherry luciferase plasmid (Addgene) [24 ...
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bioRxiv - Immunology 2024Quote: ... This backbone contains derivatives of the previously described reagents lentiCRISPR v2 (a gift from Feng Zhang, Addgene plasmid #5296166), and lentiCas9-EGFP (a gift from Phil Sharp and Feng Zhang ...
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bioRxiv - Cancer Biology 2021Quote: ... siRNAs were obtained from GE Dharmacon and transfections were performed with Lipofectamine RNAiMAX transfection reagent. CDK1 (Cat. 61840) and CDK1AF (Cat. 39872) plasmids were purchased from Addgene and transfections were performed with FuGENE 6 transfection reagent.
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bioRxiv - Genetics 2022Quote: ... We have also generated targeting fragments to insert arrays into MosTI sites that use hygromycin and Pmlc-2::gfp but have not tested these fragments (although we deposited the reagents with Addgene). The protocols are identical except for using targeting fragments and sgRNAs that are specific to each insertion site (listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5ul PLUS reagent with 1000 ng of reporter donor plasmid and 500 ng of each TALEN-L (Addgene 35431) and TALEN-R (Addgene 35432 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cloning of plasmids was done following Zhang Lab General Protocol (http://www.genome-engineering.org/crispr/wp-content/uploads/2014/05/CRISPR-Reagent-Description-Rev20140509.pdf) by using pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene plasmid #50946).
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine™ 3000 transfection reagent in Opti-MEM medium was used to co-transfect HEK293T cells with psPAX2 (#12260, Addgene, USA), pMD2.G (# 12259 ...
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bioRxiv - Cell Biology 2019Quote: ... plasmids were then transformed into the strains to enable detection of MS2 and PP7-tagged mRNAs The MS2 and PP7 tagging reagents were gifts from Jeff Gerst and Robert Singer (Addgene #31864 & #35194) (Haim-Vilmovsky and Gerst ...
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bioRxiv - Biophysics 2022Quote: ... the RNA-guided targeting of genes was achieved through coexpression of the Cas9 protein with gRNAs using reagents from the Church group48 (Addgene: http://www.addgene.org/crispr/church/). Targeting sequences of the gRNAs were as followed ...