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Citations for Addgene :
1 - 40 of 40 citations for Phospho Tau antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... The plasmid expressing Tau was subcloned from pRK5-EGFP-Tau (46904, Addgene) with the Tau coding sequence replacing the EGFP-Tau fusion sequence between the restriction sites ClaI and SalI.
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bioRxiv - Neuroscience 2023Quote: ... pEGFP-WT Tau (Addgene #46904) and pEGFP-P301L tau (Addgene #46908).
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bioRxiv - Neuroscience 2024Quote: ... and pmGFP11C-Tau (Addgene, #71434), were transfected into naïve N2a cells using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment encoding human 4R1N full length tau were amplified by PCR using tau cDNA (Addgene). And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara) ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragment encoding human 4R1N full length tau were amplified by PCR using tau cDNA (Addgene). And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... and pEGFP-P301L tau (Addgene #46908).
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bioRxiv - Bioengineering 2022Quote: ... The pRK5-EGFP-tau (# 46904, RRID: Addgene_46904) encodes EGFP fused to human tau isoform 0N4R ...
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bioRxiv - Neuroscience 2024Quote: ... two plasmid constructs pmGFP10C-Tau (Addgene, #71433) and pmGFP11C-Tau (Addgene ...
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bioRxiv - Biochemistry 2019Quote: ... a mammalian expression vector pRK5 encoding EGFP tagged wild-type (WT) human tau (pRK5-EGFP-tau, # 46904, RRID: Addgene_46904), which contains four C-terminal repeat regions and lacks the N-terminal sequences (0N4R) ...
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bioRxiv - Biophysics 2020Quote: ... [24] using the plasmid tau/pET29b (Addgene, #16316) and E ...
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bioRxiv - Molecular Biology 2020Quote: FLAG-mEm-WT Tau and FLAG-mEm-Tau K174Q sequences were cloned into pAAV2 vector under human synapsin promoter (Addgene, #50465). Adenoviral particles were used to infect a primary hippocampal culture.
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bioRxiv - Neuroscience 2020Quote: Plasmid Cry2olig-mCherry-tau 1-441 was prepared by inserting DNA fragment encoding the full length tau into the linearized Cry2olig-mCherry (Addgene 60032) backbone at the C-terminus of mCherry using Gibson assembly® Cloning kit (New England BioLab Int.) ...
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bioRxiv - Biophysics 2020Quote: ... Tau/pET29b was a gift from Peter Klein (Addgene plasmid # 16316 ...
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bioRxiv - Neuroscience 2020Quote: ... with the tau expression cassette sub-cloned into pCFJ151 (Addgene) to generate MosSCI inserts at the ttTi5605 loci in C ...
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bioRxiv - Cell Biology 2022Quote: ... Tau (wt)-VC was a gift from Tiago Outeiro (Addgene plasmid no ...
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bioRxiv - Cell Biology 2022Quote: ... VN-Tau (wt) was a gift from Tiago Outeiro (Addgene plasmid no ...
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bioRxiv - Cell Biology 2022Quote: ... pRK5-EGFP-Tau AP was a gift from Karen Ashe (Addgene plasmid no ...
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bioRxiv - Neuroscience 2024Quote: ... the coding sequence of human tau isoform 0N4R was subcloned into the pJFRC7 vector (Addgene, plasmid #26220 ...
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bioRxiv - Biophysics 2019Quote: The cDNAs for protein expression in this study were as follows: human Tau-2N4R (Addgene #16316), human MAP7 (GE Dharmacon MGC Collection #BC025777) ...
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bioRxiv - Biophysics 2020Quote: ... Tau/pET29b was a gift from Peter Klein (Addgene plasmid # 16316 ; http://n2t.net/addgene:16316 ; RRID:Addgene_16316) [25].
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bioRxiv - Bioengineering 2022Quote: ... Mammalian expression plasmids pRK5-EGFP-tau and pcDNA3-GSK3b were obtained from Addgene (Watertown, MA, USA). The pRK5-EGFP-tau (# 46904 ...
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bioRxiv - Neuroscience 2020Quote: ... pRK5-EGFP-tau (plasmid # 46904) and pWZL Neo Myr Flag TBK1 (plasmid # 20648) were purchased from Addgene. The TBK1 Open Reading Frame (ORF ...
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bioRxiv - Neuroscience 2022Quote: ... a mammalian expression vector encoding an EGFP-human 0N4R tau fluorescent fusion protein was obtained from Addgene (catalog # 46904). This plasmid was developed by the lab of Karen Ashe [16] ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently co- transfected for 24 h with plasmids for expression of full-length, N-terminal tagged (Flag, HA or tandem Strep tag) BRSK1/2 (or Cys-Ala mutants) and GFP-TAU (Addgene), using 3:1 polyethylenimine (average Mw ...
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bioRxiv - Cell Biology 2019Quote: Plasmids for the FRET-based aggregation reporter were constructed by cloning a fusion of the K18 repeat domain of tau containing the P301L/V337M mutation (20) in frame with C-terminal Clover2 (Addgene #54711) or mRuby2 (Addgene #54768 ...
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bioRxiv - Neuroscience 2020Quote: ... we cloned WT and mutant forms of the TSC1 and MAPT/tau genes in the pLenti-CMV plasmid (Addgene plasmid # 17392) (71) ...
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bioRxiv - Cell Biology 2021Quote: ... sequence from N1-TauRD (P301L/V337M)-EYFP and N1-TauRD (P301L/V337M)-mTurquoise2 plasmids by the FLTau (0N4R, P301L/V337M) sequence from the pRK5-EGFP-Tau P301L plasmid (a gift from Karen Ashe (Addgene #4690881) after introducing the mutation V337M ...
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bioRxiv - Cell Biology 2022Quote: ... VN-Tau (wt) was a gift from Tiago Outeiro (Addgene plasmid no. 87368; http://n2t.net/addgene:87368; RRID:Addgene_87368; (Blum et al., 2015). Tau (wt)-VC was a gift from Tiago Outeiro (Addgene plasmid no ...
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bioRxiv - Cell Biology 2022Quote: ... pRK5-EGFP-Tau AP was a gift from Karen Ashe (Addgene plasmid no. 46905; http://n2t.net/addgene:46905; RRID:Addgene_46905; (Hoover et al., 2010).
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bioRxiv - Cell Biology 2022Quote: ... Tau (wt)-VC was a gift from Tiago Outeiro (Addgene plasmid no. 87369; http://n2t.net/addgene:87369; RRID:Addgene_87369; (Blum et al., 2015). pRK5-EGFP-Tau AP was a gift from Karen Ashe (Addgene plasmid no ...
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bioRxiv - Cell Biology 2021Quote: The Tau/pET29b plasmid used for wild type FLTau (2N4R) expression and purification was a gift from Peter Klein (Addgene plasmid #1631683).
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bioRxiv - Neuroscience 2023Quote: ... Plasmids used for tdTomato-Tau overexpression and the tdTomato-C1 control plasmid were a gift from Michael Davidson: tdTomato-MAPTau-C-10 (Addgene plasmid #58112), tdTomato-MAPTau-N-10 (Addgene plasmid #58113 ...
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bioRxiv - Neuroscience 2024Quote: ... the coding sequence of human tau isoform 0N4R was subcloned into the pJFRC7 vector (Addgene, plasmid #26220. http://n2t.net/addgene:26220; RRID:Addgene_26220, a gift from Gerald Rubin43) to allow expression under the control of the GAL4/UAS system ...
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bioRxiv - Cell Biology 2021Quote: ... Single-chain antibodies fused to GFP (scAB-GFP, Addgene #104998) were used for imaging translation sites through nascent polypeptide labeling ...
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bioRxiv - Immunology 2024Quote: ... VDJ sequences were ordered from IDT and cloned into the vector AbVec antibodies (Addgene)
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bioRxiv - Bioengineering 2020Quote: ... Heavy and light chain DNA sequences of antibody fragments (Fab) were purchased from Twist Bioscience and cloned separately into the pHLsec mammalian expression vector (Addgene, #99845) via Gibson assembly ...
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bioRxiv - Microbiology 2021Quote: ... the antibody component of the SunTag system (pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS45, a gift from Ron Vale, Addgene #60904) was digested with EcoRI+NotI and subcloned into the blunted BamHi site of pCW-TRE ...
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bioRxiv - Bioengineering 2023Quote: ... To generate the monoclonal IgG1 antibody CSL362 biosimilar MIRG123 the VH and VKL sequences were cloned into AbVec2.0-IGHG1 (Addgene plasmid # 80795) and AbVec1.1-IGKC (Addgene plasmid # 80796) ...
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bioRxiv - Cell Biology 2020Quote: The ATF4-SunTag reporter was stably integrated into a previously-described HeLa-11ht cell line stably expressing GFP-tagged single-chain antibodies (scFvGFP) against GCN4 (Addgene plasmid #104998) and NLS-stdMCP-stdHalo fusion proteins (Addgene plasmid #104999 ...
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bioRxiv - Genetics 2024Quote: To generate the B16F10 Capza3 KO lines the 2 CRISPR gRNAs enriched in the radiation alone and radiation and anti-PD1 antibody screen treatment conditions were cloned into the LRG vector (Addgene Plasmid # 65656). For WT controls a NTC gRNA from the CRISPR screen was cloned into the LRG vector ...