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Citations for Addgene :
1 - 46 of 46 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... pTG023 was constructed using PCR amplification of Plas-gRNA-LEU (Addgene #309094), a gBlock designed to fuse csGBP-mAID (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... A plasmid expressing 8X-His-TEV-8X-Arg tag protease was obtained from Addgene and purified according to the published protocol (Tropea et al. ...
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bioRxiv - Cancer Biology 2021Quote: Previously described pMIG-Aalpha WT retroviral vector with a Flag-tag at the NH2 terminal (32) obtained from Addgene (plasmid #10884) was generously provided by William Hahn ...
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bioRxiv - Molecular Biology 2023Quote: ... H-RASV12 from pBABE-Puro H-RASV12 (n°12545 Addgene) was cloned in a home-made inducible vector derived from pLVX-Tight-Puro (Clontech ...
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bioRxiv - Microbiology 2023Quote: ... pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586 ...
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bioRxiv - Neuroscience 2023Quote: ... the signal peptide (0-26 amino acids) of pro-opiomelanocortin (#176704, Addgene) and ectodomain (52-750 amino acids ...
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bioRxiv - Biochemistry 2024Quote: ... was replaced with H-Ras V12 cDNA from pBABE-puro H-Ras V12 (Addgene #9051) to generate pWZL H-Ras V12 blast.
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bioRxiv - Cancer Biology 2020Quote: ... H-Ras V12 (Addgene plasmid # 18749), and ST (Addgene plasmid # 8583 ...
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bioRxiv - Cell Biology 2020Quote: ... h-lin-28 and EBNA-1 (Addgene plasmids #27077 ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Neuroscience 2022Quote: ... 72-h after co-transfection with PHP.eB (Addgene, 103005), pAdDeltaF6 (Addgene ...
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bioRxiv - Molecular Biology 2022Quote: Human Reg3α (hReg3α) lacking the N-terminal inhibitory pro-peptide was expressed and purified from a pET3a vector (Addgene plasmid ID 64937 ...
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bioRxiv - Microbiology 2023Quote: ... pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586; http://n2t.net/addgene:138586; RRID: Addgene_138586). All the plasmids used in this study were verified by Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... The extracellular ADAM17 pro- and metalloprotease domains (residues 1– 477) were cloned into the pLib vector (Addgene: Plasmid #80610), which includes a 6x HisTag at the carboxyl terminus 76 ...
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bioRxiv - Cancer Biology 2022Quote: ... pmScarlet-H-C1 was a gift from Dorus Gadella (Addgene plasmid # 85043). pEGFP-puro was a gift from Michael McVoy (Addgene plasmid # 45561) ...
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bioRxiv - Cancer Biology 2022Quote: ... pTag-RFP-C-h-Rab11a-c-Myc was a gift from James Johnson (Addgene plasmid #79806 ...
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bioRxiv - Cell Biology 2020Quote: ... Murine PtcΔL2 was generated from Ptc Full Length (pcDNA-h-mmPtch1-FL, Addgene #120889) by deletion of the second extracellular loop (L2 ...
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bioRxiv - Bioengineering 2024Quote: ... and this construct was termed DoubleCatcher H-Lock (GenBank and Addgene deposition in progress). Double-cysteine substitution variants of DoubleCatcher were derived from pDEST14-SpyCatcher003-(GSG)3-SpyCatcher003-TEVs-SpyTag003DA by Gibson assembly ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h cells were co-transfected with 1.3 pmol of psPAX2 (Addgene, 12260), 0.72 pmol pCMV-VSV-G (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... cultured for 24 h and transfected with either pWPI-eGFP (Addgene, 12254, from Didier Trono) or pWPI-eGFP-NDI138 constructs with the with the Lipofectamine® 2000 transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Overnight (∼16 h) cultures were transfected with lentiviral packaging plasmids pMD2.G and psPAX2 (Addgene plasmid # 12259 and 12260 ...
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bioRxiv - Cell Biology 2023Quote: ... H-2Kb in the pSBbi-puromycin vector was obtained from Addgene (deposited by Jonathan Yewdell). APOL7C::mCherry was synthesized by BioBasic in a pcDNA3.1(+ ...
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bioRxiv - Neuroscience 2020Quote: The original AAV-NKCC1 plasmid was purchased from Addgene (pcDNA3.1 HA CFP hNKCC1 WT (NT15-H) was a gift from Biff Forbush ...
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bioRxiv - Cell Biology 2024Quote: ... Murine Ptch1tg and Ptch1ΔL2 were generated from Ptch1 Full Length (pcDNA-h-mmPtch1-FL, Addgene #120889). Ptch1ΔL2 was generated by deletion of the second extracellular loop (L2 ...
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bioRxiv - Biophysics 2024Quote: ... MEFs were transfected 24 h before the experiment with the mScarlet-PM (Lck) plasmid (Addgene: 98821). Before the experiment ...
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bioRxiv - Neuroscience 2021Quote: ... mice were microinjected 0.3 µl of AAV5-hsyn-NE2.1 (h-N01, WZ Biosciences) and 0.5 µl of AAV9-hsyn-jRGECO1a (100854, Addgene) to the left Cg1 (AP=2.2mm ...
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bioRxiv - Cancer Biology 2024Quote: ... hGli2 FLAG3x was a gift from Martin Fernandez-Zapico (Addgene plasmid # 84920; h-p://n2t.net/addgene:84920; RRID:Addgene_84920). The plasmid backbone sequence (p3xFLAG-CMV-14 ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were digested with 2.0 µL of PNGase F for 20 h at 37°C (in-house, Addgene #114274 ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixes were prepared in MilliQ H O and contained 50 ng/ml Peft-3::cas9 (Addgene ID #46168) (Friedland et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled clones were expanded before being transfected with Not1-linearized pWZL-hygro H-Ras V12 plasmid (Addgene plasmid # 18749) and selected with hygromycin for 2-4 weeks to generate stable clone ...
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bioRxiv - Cancer Biology 2022Quote: ... pTag-RFP-C-h-Rab11a-c-Myc was a gift from James Johnson (Addgene plasmid #79806; http://n2t.net/addgene:79806; RRID: Addgene_79806)66 ...
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bioRxiv - Cell Biology 2024Quote: pORANGE-mEGFP-Palm1 was based on the CRISPR/Cas9 knock-in template vector pORANGE (gift from H. MacGillavry, Addgene plasmid #131471; RRID:Addgene_131471)55 ...
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bioRxiv - Cancer Biology 2021Quote: Human H-RasG12V cDNA sequence was cloned into LeGO-iV2 and LeGO-iC2 bicistronic vectors (Addgene #27344 and #27345, respectively). Retroviruses were produced by JetPEI (Polypus-Transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... we first generated OsTIR1 parental cell line (Supplementary Fig. 1G, H) by transfecting pEN396-pCAGGS-Tir1-V5-2A-PuroR (Tigre donor, #92142, Addgene) and pX330-EN1201 (spCas9nuclease with Tigre sgRNA ...
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bioRxiv - Cell Biology 2023Quote: HEK293T-LentiX cells were co-transfected for 12 h with 10 µg psPAX2 (kind gift from Didier Trono, Addgene #12260), 2.5 µg pMD2.G (kind gift from Didier Trono ...
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bioRxiv - Cell Biology 2023Quote: ... Sequences encoding N-KSR or C-KSR were subcloned into SfiI sites of pSBbi-pur-H-2Kb (# 111623, Addgene, USA) replacing an existing H-2kb fragment ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded at 30 000 cells/cm2 and transfected 24 h after with the calcium reporter pCAG mito-GCaMP5G, a generous gift from Franck Polleux (Kwon et al., 2016) (Addgene plasmid # 105009 ...
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bioRxiv - Microbiology 2024Quote: ... BHK-21/WI-2 culture supernatants were filtered (0.22 μm) and used to inoculate 293T cells that had been transfected 24 h previously with pMD2.G (Addgene 12259). At 24 h post-inoculation ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 million cells per replicate were activated and 12-20 h later electroporated with the pSpCas9(BB)-2A-GFP plasmid (pX458; Addgene 48138) expressing the Myc sgRNA using the MaxCyte STx transfection system (MaxCyte) ...
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bioRxiv - Cancer Biology 2021Quote: MEFs were seeded in 6-well plates and transfected for 6-8 h with 1 μg of plasmid 4XCLEAR-luciferase reporter (Addgene, 66800) and 0.1 ug of CMV-Renilla Luciferase (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... pUC19 was purchased from New England Biolabs and pJKR-H-araC [25] was a gift from George Church (Addgene plasmid # 62563). Plasmids pEX04 ...
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bioRxiv - Cell Biology 2020Quote: ... and transfected at 4-6 h with a plasmid encoding HA-Separase (pCS2+HA-hSeparase was a gift from Marc Kirschner, Addgene plasmid # 33018), Plk1TD expression was induced 8-10 h after shake off and cells were fixed at 28 h to analyze distancing or at 32 h (20 h of S phase arrest ...
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bioRxiv - Biophysics 2022Quote: ... lentivirus was generated using pcDNA/TO/GFP H-Ras V12 (gift of Yasuyuki Fujita)[43] and pLenti CMV TetR Blast (Addgene plasmid 17492), and cells were selected based on fluorescent expression.
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bioRxiv - Molecular Biology 2023Quote: ... values were converted into copy numbers by measuring against a standard curve of the AAV transfer plasmid or the Ai9 plasmid (a gift from H. Zeng Addgene plasmid # 22799). The AAV genome copy number was divided by the number of nuclei to normalize for tissue input.
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bioRxiv - Cell Biology 2024Quote: pORANGE-mEGFP-Palm1 was based on the CRISPR/Cas9 knock-in template vector pORANGE (gift from H. MacGillavry, Addgene plasmid #131471; RRID:Addgene_131471)55 ...
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bioRxiv - Cell Biology 2024Quote: ... the Rap1-binding domain (RBD) of RALGDS (aa 793-914) was PCR amplified from pET28-MHL-His-RALGDS: N793-F914 (H. sapiens RALGDS, Addgene #25331, deposited by Cheryl Arrowsmith), cloned into a UASp N-terminal msVenus backbone using Gibson assembly ...