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151 - 200 of 2479 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020

• Extensive in vitro and in vivo protein translation via in situ circularized RNAs

  Aditya Kumar, et al., bioRxiv - Bioengineering 2022

  Quote: ... 3 μg pMD2.G (Addgene #12259), 12 μg pCMV delta R8.2 (Addgene #12263) ...
• The endoplasmic reticulum adopts two distinct tubule forms

  Bowen Wang, et al., bioRxiv - Cell Biology 2021

  Quote: ... mEmerald-ER-3 (Addgene plasmid #54082), and calnexin-mEmerlad (Addgene plasmid #54021 ...
• The variant Senescence-Associated-Secretory-Phenotype induced by centrosome amplification constitutes a pathway that activates Hypoxia-Inducible-Factor-1α

  Selwin K. Wu, Juliana Ariffin, Remigio Picone, bioRxiv - Cell Biology 2021

  Quote: ... and 3 μg pMD2.G (Addgene) were transfected into 293T cells at 80% confluency in a 10-cm dish with 8 ml of media ...
• A multimodal 3D neuro-microphysiological system with neurite-trapping microelectrodes

  Beatriz Molina-Martínez, et al., bioRxiv - Neuroscience 2021

  Quote: ... 3 μg psPax vector (Addgene #12260), 1.5 μg pMD2.G vector (Addgene #12259 ...
• Reactive oxygen species prevent lysosome coalescence during PIKfyve inhibition

  Golam T. Saffi, et al., bioRxiv - Cell Biology 2021

  Quote: ... or Galectin-3-GFP (Addgene; [48]). The bacterial expression vector pZsGreen (Takara Bio USA ...
• Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5

  Momoe Nakajo, et al., bioRxiv - Cell Biology 2021

  Quote: ... pCFJ104 (Pmyo-3::mCherry, Addgene #19328) and pGH8 (Prab-3::mCherry ...
• A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes

  Karen Julie la Cour Karottki, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 µg pMD2.G (Addgene #12259) and 9 µg lentiviral vector were diluted in 1.2 mL OptiMEM ...
• Phosphorylation of the RB C-terminus regulates condensin II release from chromatin

  Seung J. Kim, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and 3 μg psPAX2 (Addgene #12260) using Lipofectamine 3000 (Life Technologies ...
• Role of histone variants H2BC1 and H2AZ.2 in H2AK119ub nucleosome organization and Polycomb gene silencing

  Xiangyu Shen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 3 µg of psPAX2 (Addgene #12260), and 1.5 µg of the pMD2.G plasmid (Addgene #12259 ...
• GTPase-activating protein ARAP1 regulates circular dorsal ruffles as a nutrient uptake mechanism in the Hep3B hepatocellular carcinoma cell line

  Xiaowei Sun, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 3 μg psPAX2 (AddGene 12260) using Lipofectamine 2000 (Invitrogen ...
• FITM2 deficiency results in ER lipid accumulation, ER stress, reduced apolipoprotein B lipidation, and VLDL triglyceride secretion in vitro and in mouse liver

  Haizhen Wang, et al., bioRxiv - Biochemistry 2023

  Quote: ... mCherry-ER-3 (Addgene, Watertown, MA), or Perilipin1-YFP[41] following established protocols[32] ...
• The Senescence-Associated-Secretory Phenotype constituting HIF-1α activation is independent of micronuclei induction and associated cGAS-mediated interferon response

  Deng Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 3 μg pMD2.G (Addgene) were transfected into 293T cells at 80% confluency in a 10-cm dish with 8 ml of media ...
• Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger Cyclin K degradation

  Lu Lv, et al., bioRxiv - Biochemistry 2020

  Quote: An sgRNA sequence targeting NRF2 (5’-TATTTGACTTCAGTCAGCGA-3’) was cloned into the lentiCRISPR v2 vector (Addgene #52961). Lentiviral packaging was performed by co-transfecting the resulting plasmid with psPAX2 (Addgene 12260 ...
• MYC promotes immune-suppression in TNBC via inhibition of IFN signaling

  Dario Zimmerli, et al., bioRxiv - Cancer Biology 2021

  Quote: ... #2: 5’-caccgTGAAACGGATTCTTCTTTCG-3’) and cloned into the Cas9 plasmids pSpCas9(BB)−2A-Puro (PX459, Addgene #62988) and pSpCas9(BB)-2A-GFP (PX458 ...
• β1 integrin signaling governs necroptosis via the chromatin remodeling factor CHD4

  Zhiqi Sun, et al., bioRxiv - Immunology 2023

  Quote: ... Gatad2b (5’-CGTCTAGCACTCATATCCAC-3’) were cloned into pSpCas9(BB)-2A-Puro (PX459) V2.0 (53) (Addgene plasmid #62988). SgRNAs for CRISPR silencing (sgRipk3 enhP #1 ...
• Human Immunodeficiency Virus 1 Capsid Uncoating in the Nucleus Progresses Through Defect Formation in the Capsid Lattice

  Levi B. Gifford, Gregory B. Melikyan, bioRxiv - Microbiology 2023

  Quote: ... reverse primer: 5’ – GAGTCGggatccACTAGTAGTTCCTGCTATGTCACTTCCCCTTGG – 3’) and ligating the domain into the pUC19 cloning vector (Addgene plasmid # 50005), using the T4 ligase ligation kit (New England Biolabs ...
• Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

  Josh P. Graham, et al., bioRxiv - Bioengineering 2024

  Quote: ... pGEM4Z-T7-5’UTR-EGFP-3’UTR-A64 was a gift from Christopher Grigsby & Molly Stevens (Addgene plasmid # 203348 http://n2t.net/addgene:203348 ...
• Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2024

  Quote: ... we expressed the GST-tagged WIPI1/2d/3/4 from a pCAG backbone encoding GST-TEV-WIPI1/2/3/4 (RRID:Addgene_223798; RRID:Addgene_223799 ...
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#843: hrp-1p∷hrp-1HsLCΔLC∷hrp-1 3’UTR (Addgene ID: 139200)
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#849: mec-4p∷hrp-1HsLCD290VmScarlet∷hrp-1 3’UTR (Addgene ID: 139203)
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#841: hrp-1p∷hrp-1HsLCWT∷hrp-1 3’UTR (Addgene ID: 139198)
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#848: mec-4p∷hrp-1HsLCWTmScarlet∷hrp-1 3’UTR (Addgene ID: 139202)
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#842: hrp-1p∷hrp-1HsLCD290V∷hrp-1 3’UTR (Addgene ID: 139199)
• Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

  Veronica H. Ryan, et al., bioRxiv - Biochemistry 2020

  Quote: pHA#847: mec-4p∷hrp-1mScarlet∷hrp-1 3’UTR (Addgene ID: 139201)
• Norepinephrine Signals Through Astrocytes To Modulate Synapses

  Katheryn B Lefton, et al., bioRxiv - Neuroscience 2024

  Quote: ... was co-injected at a 3:1 ratio with AAV5-gfaABC1D::mCherry (Addgene #58909-AAV5 ...
• Visualizing transcription sites in living cells using a genetically encoded probe specific for the elongating form of RNA polymerase II

  Satoshi Uchino, et al., bioRxiv - Cell Biology 2021

  Quote: ... The resulting scFv fragments were further amplified using the 5’ and 3’ primers (scFv primer_s and scFv primer_as) and cloned into the psfGFP-N1 vector (Addgene #54737 ...
• NF-κB memory coordinates transcriptional responses to dynamic inflammatory stimuli

  Andrew G. Wang, et al., bioRxiv - Systems Biology 2022

  Quote: A Myd88-targeting guide RNA (5’-TCGCGCTTAACGTGGGAGTG-3’) was cloned into the pX330 plasmid backbone (Addgene Plasmid #42230) and transfected using electroporation (Lonza ...
• Isolation of bat sarbecoviruses of SARS-CoV-2 clade, Japan

  Shin Murakami, et al., bioRxiv - Microbiology 2022

  Quote: ... The target sequence for the ACE2 gene (5′-TGCTGCTCAGTCCACCATTG-3′) was designed using CRISPR direct (https://crispr.dbcls.jp) and cloned into plentiCRISPR plasmids (21) (Addgene plasmid #52961 ...
• MLL3/MLL4 methyltransferase activities control early embryonic development and embryonic stem cell differentiation in a lineage-selective manner

  Guojia Xie, et al., bioRxiv - Molecular Biology 2022

  Quote: ... a sgRNA sequence cutting near MLL4 Y5477 (5’-ACGATGGTCATCGAGTACAT-3’) was cloned into lentiCRISPR v2 plasmid (Addgene #52961). The Tyrosine to Alanine point mutation was introduced using single-strand Ultramer DNA Oligonucleotide (IDT) ...
• Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity

  Tiffany Cho, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 4 × 106 293T cells were seeded in a 10-cm plate 1 d prior to transfection and co-tranfected with the 3rd generation lentiviral packaging plasmids (5 µg pVSV.G, 3 µg pMDLg/pRRE, and 2.5 µg pRSV-Rev; Addgene # 14888 ...
• Glia-mediated cerebellar pain modulation

  Seung Ha Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... 100-200 nl virus solution of 3-5 × 1010 genome copies containing AAV5.gfaABC1d.Lck-GCaMP6f (Addgene, MA, USA) were injected at two or three sites in the craniotomy site at the cerebellar cortex (Vermis Lobule 4/5 ...
• Multi-omics analysis reveals distinct non-reversion mechanisms of PARPi resistance in BRCA1- versus BRCA2-deficient mammary tumors

  Jinhyuk Bhin, et al., bioRxiv - Cancer Biology 2022

  Quote: ... sgRNA#2: 5’-aacctgagtgatatgactag-3’) were cloned into a modified version of the lentiCRISPR v2 backbone (RRID: Addgene_52961) in which a puromycin resistance ORF was cloned under the hPGK promoter ...
• Erythrocyte membrane protein 3 (EMAP3) is exposed on the surface of the Plasmodium berghei infected red blood cell

  Sophia Hernandez, et al., bioRxiv - Microbiology 2024

  Quote: ... The 5’ p230p and 3’ p230p targeting sequences were cloned into the pPbU6-hdhfr/yfcu plasmid (Addgene #216422),44 carrying the dual positive and negative selection marker hdhfr-yfcu (human dihydrofolate reductase/yeast cytosine deaminase and uridyl phosphoribosyl transferase ...
• Mis-regulation of GSK-3β causes axonal microtubule curling through Shot and Tau

  Andre Voelzmann, et al., bioRxiv - Cell Biology 2024

  Quote: CRISPR donor plasmids were cloned with 0.8 kb 5’ and 3’ homology arms amplified from fly genomic DNA into pUC57(Addgene plasmid #51019, RRID:Addgene_51019), flanking the different CTail Shot-eGFP coding sequences ...
• Network-level encoding of local neurotransmitters in cortical astrocytes

  Michelle K. Cahill, et al., bioRxiv - Neuroscience 2023

  Quote: ... neonatal Swiss Webster or C57Bl/6 (P0–3) mice were anesthetized on ice for 3 min before injecting viral vectors (AAV5.GfaABC1D.GCaMP6f.SV40 [Addgene, 52925-AAV5] ...
• Genetic encoding of 3-nitro-tyrosine reveals the impacts of 14-3-3 nitration on client binding and dephosphorylation

  Phillip Zhu, et al., bioRxiv - Biochemistry 2022

  Quote: ... The wild-type 14-3-3 ζ gene was cloned into NcoI/XhoI digested pBAD plasmid (Addgene #85482) with a TEV cleavable C-terminal His6 purification tag ...
• SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis

  Cecilia Colson, et al., bioRxiv - Biochemistry 2024

  Quote: ... The 3×HA and 3×HA-GFP sequences were cloned from pMXs-3XHA-EGFP-OMP25 plasmid (Addgene 83356).
• Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans

  Troy A. McDiarmid, et al., bioRxiv - Genomics 2020

  Quote: ... pCFJ104 - Pmyo-3∷mCherry∷unc-54 (Addgene plasmid # 19328 ...
• Widespread multi-targeted therapy resistance via drug-induced secretome fucosylation

  Mark Borris D. Aldonza, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and 3 μg psPAX2 (packaging plasmid; Addgene) was performed using iN-fect (Intron Biotechnology ...
• Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5

  Momoe Nakajo, et al., bioRxiv - Cell Biology 2021

  Quote: ... and pGH8 (Prab-3::mCherry, Addgene #19359) (Frøkjaer-Jensen et al. ...
• Hi-TrAC reveals fine-scale chromatin structures organized by transcription factors

  Shuai Liu, et al., bioRxiv - Genomics 2022

  Quote: ... and 3 μg pMD2.G (Addgene, #12259) into 293T cells cultured in 100 mm dish ...
• Intermitochondrial signaling regulates the uniform distribution of stationary mitochondria in axons

  Nozomu Matsumoto, et al., bioRxiv - Neuroscience 2020

  Quote: ... 3 μg of pGW-PervevalHR (Addgene #57432) (22) ...
• A FOXN1 mutation competitively displaces wild-type FOXN1 from higher-order nuclear condensates to cause immunodeficiency

  Ioanna A. Rota, et al., bioRxiv - Immunology 2021

  Quote: ... myc (pCSF107mT-GATEWAY-3’-Myc tag, Addgene), green fluorescence protein (GFP ...
• High throughput PRIME editing screens identify functional DNA variants in the human genome

  Xingjie Ren, et al., bioRxiv - Genomics 2023

  Quote: ... with 3 μg of psPAX (Addgene, 12260) and 1 μg of pMD2.G (Addgene ...
• Direct conversion of somatic cells into ‘insulin-producing-cells’ by user-defined multiplex-epigenetic-engineering vector (MEEV-β)

  Raza Ali Naqvi, et al., bioRxiv - Synthetic Biology 2022

  Quote: ... MAFA in pX330S-3 (Plasmid #58779, Addgene), Insulin in pX330S-4 (Plasmid #58780 ...
• PARP14 inhibition restores PD-1 immune checkpoint inhibitor response following IFNγ-driven adaptive resistance

  Chun Wai Wong, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and 3 μg of pVSVg (8454; Addgene); FuGENE HD transfection reagent (Promega ...
• Efficient hyperactive piggyBac transgenesis in Plodia pantry moths

  Christa Heryanto, Anyi Mazo-Vargas, Arnaud Martin, bioRxiv - Genetics 2022

  Quote: ... Tc’hsp5’-Gal4Delta-3’UTR] (Addgene plasmid # 86449) was used as a donor plasmid with Piggybac insertion repeats and the 3xP3::EGFP reporter (Schinko et al. ...
• The Small GTPase Rab7 Regulates Release of Mitochondria in Extracellular Vesicles in Response to Lysosomal Dysfunction

  Wenjing Liang, et al., bioRxiv - Physiology 2023

  Quote: ... and mPlum-mito-3 (Addgene plasmid #55988) using Fugene6 (Promega Inc. ...
• An Atlas of Protein Phosphorylation Dynamics During Interferon Signaling

  Idoia Busnadiego, et al., bioRxiv - Immunology 2024

  Quote: ... pcDNA-HA-14-3-3β (Addgene #13270), pcDNA-HA-14-3-3σ (Addgene #11946 ...
• An Atlas of Protein Phosphorylation Dynamics During Interferon Signaling

  Idoia Busnadiego, et al., bioRxiv - Immunology 2024

  Quote: ... pCS2-HA-14-3-3ε (Addgene #116886), pCS2-HA-14-3-3ζ (Addgene #116888 ...