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1 - 10 of 10 citations for Recombinant Human CD3E & CD3D His & Flag tag since 2019

• Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactors

  Guangda Shi, et al., bioRxiv - Cell Biology 2021

  Quote: ... 50 μM Phos-Tag reagent (Nard Institute AAL-107), and 100 μM MnCl2 ...
• Intrinsically disordered proteins SAID1/2 condensate on SERRATE/ARS2 for dual inhibition of miRNA biogenesis in Arabidopsis

  Baoshuan Shang, et al., bioRxiv - Plant Biology 2022

  Quote: ... The lysates were subjected for Phos-tag™ SDS-PAGE analysis following guidelines of the manufacture (Nard Institute, LTD, Phos-tag™ Acrylamide AAL-107M).
• Regulating peroxisome-ER contacts via the ACBD5-VAPB tether by FFAT motif phosphorylation and GSK3β

  Suzan Kors, et al., bioRxiv - Cell Biology 2021

  Quote: ... 50 μM Phos-tag acrylamide (Wako Chemicals, purchased from NARD Institute ltd.) and 100 μM MnCl2 were added to the resolving gel solution of 8% SDS-PAGE gels before polymerization ...
• The p21 dependent G2 arrest of the cell cycle in epithelial tubular cells links to the early stage of renal fibrosis

  Takayuki Koyano, et al., bioRxiv - Cell Biology 2019

  Quote: ... phosphate affinity SDS-PAGE was carried out by using Phos-tag acrylamide (NARD Institute) (39).
• Distinct phosphorylation signals drive acceptor versus self-ubiquitination selection by Parkin

  Karen M. Dunkerley, et al., bioRxiv - Biochemistry 2021

  Quote: ... Protein phosphorylation was monitored to completion using Phos-Tag AAL™ (NARD Institute Ltd) SDS–PAGE ...
• The Hippo kinase LATS1 controls CTCF chromatin occupancy and the hormonal response of three-dimensionally grown breast cancer cells

  Julieta Ramirez, et al., bioRxiv - Genomics 2023

  Quote: Whole cell lysates were separated on a 5% SDS/PAGE containing 20 μm Phos-tag (NARD Institute), followed by western blotting with anti-CTCF antibody.
• MPK4 mediated phosphorylation of PIF4 controls thermosensing by regulation of H2A.Z deposition in Arabidopsis

  Neetu Verma, et al., bioRxiv - Plant Biology 2023

  Quote: In Vivo phosphorylation of PIF4 protein was examined by mobility sift assay using the Phos-tag reagent (NARD Institute) as described previously (Li et al. ...
• Arabidopsis Casein Kinase 2 triggers Stem Cell Exhaustion under Al Toxicity and Phosphate Deficiency Through activation of the DNA Damage Response pathway

  Pengliang Wei, et al., bioRxiv - Plant Biology 2020

  Quote: ... Proteins (1 μg) were separated on an 8% SDS-PAGE gel containing 40 μM MnCl2 and 20 μM Phos-tag (NARD Institute), which was used for identification of phosphorylated SOG1 protein ...
• Linear motif specificity in signaling through p38α and ERK2 mitogen-activated protein kinases

  Jaylissa Torres Robles, et al., bioRxiv - Cell Biology 2022

  Quote: ... Samples were boiled at 95ºC for 5 min and fractionated on 5% SDS-polyacrylamide gels with 25 nM Phos-tag reagent (Nard Institute AAL-107) and 50 μM MnCl2 as reported previously78 or by standard SDS-PAGE ...
• Bistable, biphasic regulation of PP2A-B55 accounts for the dynamics of mitotic substrate phosphorylation

  Julia Kamenz, et al., bioRxiv - Cell Biology 2020

  Quote: ... or in the case of the phosphorylated species of Arpp19 and ENSA on a 10% Tris-HCl SDS-PAGE supplemented with 10 μM Phos-tag reagent (NARD Institute, AAL-107) and then transferred onto PVDF membranes ...