Rapamycin (Sirolimus, RAPA, Rapamune, AY-22989, NSC 226080, LCP-Siro, SILA 9268A, CAS 53123-88-9), >99%
LC Laboratories' Product Number R-5000 - Rapamycin (Sirolimus, RAPA, Rapamune, AY-22989, NSC 226080, LCP-Siro, SILA 9268A), >99% - for research use only. Immunosuppressant, related to FK-506, but without calcineurin inhibitory activity even when complexed to FK-506 binding protein. Selectively blocks signaling that leads to p70 S6 kinase activation (IC50 = 50 pM). Lymphokine-induced cell proliferation at the G1 phase is inhibited and apoptosis in a murine B cell line is induced by rapamycin. Rapamycin arrests the Saccharomyces cerevisiae cell cycle irreversibly in the G1 phase. Due to a different mechanism of action than cyclosporin and FK506, rapamycin may prove to be important in organ transplant patient therapy. Fewer side effects than the standard anti-rejection treatments have been observed. Proliferation of activated T cells, but not apoptosis, is blocked by rapamycin. The induction of apoptosis of rejection-causing T cells reduces the tendency towards transplant rejection. Quantitation of rapamycin, metabolites and related compounds such as everolimus and FK-506 in blood, tissues and other matrices is offered as a service by Rocky Mountain Labs. This firm is not affiliated with LC Labs.
| Supplier | LC Laboratories |
|---|---|
| Product # | R-5000 |
| Sku # | R-5000_2g |
| Pricing | 2 g, $628.00 |
Citations for this product:
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bioRxiv - Neuroscience 2020Quote: ... Rapamycin (LC Laboratories # R-5000) was prepared as described previously (Beirowski et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μM rapamycin (LC Laboratories, R-5000) stock in DMSO was diluted in extracellular medium and used at final concentration of 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Rapamycin (R-5000, LC Laboratories) treatment was with 2.5 μg/mL for 45 minutes prior to the start of imaging.
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bioRxiv - Neuroscience 2020Quote: ... Rapamycin was purchased from LC laboratories (R-5000). Haloperidol was initially dissolved in glacial acetic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... mice received daily intraperitoneal injections with rapamycin (LC Laboratories, R-5000) (2 mg kg-1 per day ...
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bioRxiv - Cell Biology 2020Quote: ... 100mM Rapamycin stock in DMSO (LC labs # R-5000) was diluted 100 folds by mixing 890ul PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Rapamycin (LC Laboratories R-5000) was dissolved in DMSO and administered for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: Rapamycin (5 mg/kg; LC Laboratories, Cat.#: R-5000) was freshly dissolved in vehicle solution (100% DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... Rapamycin used to inhibit mTOR activity was purchased from LC Laboratories (R-5000). Thymidine to arrest cell cycle was purchased from Sigma-Aldrich (T1895).
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bioRxiv - Microbiology 2019Quote: ... 200 µl of sample were prepared: 10,000 spores were resuspended in 198 µL of malt 1 % and 2 µL of rapamycin (LC laboratories, R-5000) were added for treatment or 2 µl of DMSO as mock for control ...