| Supplier |
CD BioSciences |
| Product # |
AMA-0019 |
| Pricing |
100 μL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB
|
| Product Usage Information Dilution |
Western Blotting (1:1000)
|
| Reactivity |
Human, Monkey, Mouse, Rat
|
| Host |
Mouse
|
| Gene ID |
3667
|
| UniProt ID |
|
| Source/Isotype |
Mouse IgG1
|
| MW (Target) |
180 kDa
|
| Storage |
Store at -20°C.
|
| Purification |
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser612 of human IRS-1.
|
| Background |
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
|