| Supplier |
CD BioSciences |
| Product # |
AMA-0562 |
| Pricing |
100 µL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IP
|
| Product Usage Information Dilution |
Western Blotting (1:2000); Immunoprecipitation (1:50)
|
| Reactivity |
Human, Mouse, Rat
|
| Host |
Rabbit
|
| Gene ID |
219699
|
| UniProt ID |
|
| Source/Isotype |
Rabbit IgG
|
| MW (Target) |
130 kDa
|
| Storage |
Store at -20°C. Do not aliquot the antibody.
|
| Purification |
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human UNC5B protein.
|
| Background |
Netrin proteins belong to an evolutionarily conserved family of laminin-like molecules that are involved in axon guidance and vascular development. These secreted proteins can have opposing functions depending on specific receptor association. For example, deleted in colorectal cancer (DCC) family receptors typically mediate cellular attraction while netrin bound to UNC5 family receptors induce cellular repulsion The uncoordinated 5B homolog (UNC5B) is a transmembrane protein with extracellular Ig-like domains and an intracellular region containing a protein-binding death domain and a putative DCC interaction domain Homodimers composed of DCC receptor proteins mediate axonal attraction responses, while UNC5B homodimers and UNC5B-DCC heterodimers promote cellular repulsion The netrin receptor UNC5B mediates apoptosis in the absence of netrin through the activation of DAP kinase and is involved in leukocyte migration inhibition Expression of UNC5B correlates with bladder cancer stage and the receptor is a potential predictor of both bladder and colorectal cancer prognosis and possible disease recurrence.
|