WesternBright ECL Spray-HRP Substrate
Strong, long-lasting signal and low background are signatures of Advansta’s WesternBright HRP substrates for chemiluminescent Western blotting. Consume less antibody, use shorter exposure times, and image blots hours after substrate incubation. WesternBright ECL is an economical choice for routine blotting. WesternBright Quantum provides a large linear dynamic range with no band “burn out” (negative banding) at high protein loads. WesternBright Sirius, the most sensitive, detects bands not seen with other substrates. All are compatible with film detection and CCD imaging.
| Supplier | Advansta Corporation |
|---|---|
| Product # | K-12049-D50 |
| Pricing | 2x250 ml (for 5000 cm² of membrane), USD $324.00/ea |
Citations for this product:
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bioRxiv - Molecular Biology 2019Quote: ... and chemiluminescence was detected using Western Bright ECL spray (Advansta, #K-12049-D50). Detection of TPP1 was performed using a renaturation protocol as described (Loayza & De Lange ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... The blot was visualized with ECL reaction (#K-12049-D50, Advansta). The images were obtained with a Chemidoc imager (Bio Rad ...
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bioRxiv - Biophysics 2022Quote: ... blots were developed using WesternBright ECL-spray (Advansta K-12049-D50) and labForce HyBlot CL Autoradiography Film (Thomas Scientific 1141J52) ...
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bioRxiv - Cell Biology 2023Quote: ... and developed using westernBright ECL-spray (K-12049-D50, Advansta) and a CURIX 60 developer (Agfa Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... Reactivity was detected using Western Bright ECL spray device (cat#K-12049-D50, Advansta, Aurogene) and Fluorchem E (ProteinSimple) ...