Phospho-HS1 (Tyr397) Antibody

• Supplier: CD BioSciences
• Product #: AMA-0386
• Product Category: Antibodies
• Application: WB, IP
• Product Usage Information Dilution: Western Blotting (1:1000); Immunoprecipitation (1:50)
• Reactivity: Human
• Gene ID: 3059
• MW (Target): 80 kDa
• Storage: Store at -20°C. Do not aliquot the antibody.
• Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human HS1. Antibodies are purified using peptide affinity chromatography
• Background: HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin HS1 contains four cortactin repeats and a single SH3 domain This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 HS1 also plays an important role in platelet activationTyr397 of HS1 protein is rapidly phosphorylated by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation.