CDK4 (D9G3E) Rabbit Antibody (BSA and Azide Free)

Supplier CD BioSciences
Product # AMA-0420
Pricing 100 µg, Inquire
Product Category Antibodies
Application WB, IHC, IF, F
Reactivity Human, Monkey
Host Rabbit
Gene ID 1019
UniProt ID
Source/Isotype Rabbit IgG
MW (Target) 30 kDa
Storage Store at -20°C. Do not aliquot the antibody.
Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK4 protein.
Background Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle.
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