N-Cadherin Protein, Human, Recombinant (His Tag)

Supplier Ace Therapeutics
Product # NSC1336
Pricing Inquiry
Product Size 100 µg
Purity > 90 % as determined by SDS-PAGE
Product Type Proteins
Synonyms CD325 Protein, Human; CDHN Protein, Human; CDw325 Protein, Human; NCAD Protein, Human
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Activity Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 104 cells/well are added to CDH2 coated plates (10 μg/mL with 100 μL/well), approximately 60% will adhere after 1 hour at 37℃.
Protein Construction A DNA sequence encoding the extracellular domain of human CDH2 (NP_001783.2) precursor (Met 1-Ala 724) was fused with a polyhistidine tag at the C-terminus.
Accession# NP_001783.2
Expressed Host HEK293 Cells
Species Human
Predicted N Terminal Ser 26
Molecule Mass The pro form of human CDH2 consists of 710 amino acids and predictes a molecular mass of 78.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of CDH2 is 90 & 75 kDa corresponding to the pro fom and mature form respectinely due to glycosylation.
Formulation Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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