| Supplier |
Ace Therapeutics |
| Product # |
NSC1150 |
| Pricing |
Inquiry
|
| Product Size |
50 µg
|
| Purity |
> 95 % as determined by SDS-PAGE
|
| Product Type |
Proteins
|
| Synonyms |
CED Protein, Human; DPD1 Protein, Human; LAP Protein, Human; TGF-beta 1 Protein, Human; TGFB Protein, Human; TGFbeta Protein, Human
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method
|
| Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc, the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL.
|
| Protein Construction |
A DNA sequence encoding the full length of human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag.
|
| Accession# |
NP_000651.3
|
| Expressed Host |
HEK293 Cells
|
| Species |
Human
|
| Predicted N Terminal |
Leu 30 & Ala 279
|
| Molecule Mass |
The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.
|
| Formulation |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
|
| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|
| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
|