C57BL/6J-Tg(CAG-LSL-dCas9-SPH) Mouse
Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice. SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher).
| Supplier | Ace Therapeutics |
|---|---|
| Product # | DMM-001112 |
| Pricing | Inquiry |
| Category | Mouse Models |
| disease type | T1DM,T2DM |
| Strain Name | C57BL/6J-Tg(CAG-LSL-dCas9-SPH) |
| Background Strain | C57BL/6J |
| SPF | Yes |
| Research Application | Gene regulation tool; CRISPRa. |