KGF/FGF-7 Protein, Human, Recombinant (His Tag), HPLC-verified

Supplier Ace Therapeutics
Product # NSC1078
Pricing Inquiry
Product Size 20 µg
Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Product Type Proteins
Synonyms HBGF-7 Protein, Human; KGF Protein, Human
Endotoxin < 0.1 EU per μg of the protein.
Activity Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 3-20 ng/mL.
Protein Construction A DNA sequence encoding the human KGF (P21781) (Cys32-Thr194) was expressed with a polyhistidine tag at the N-terminus.
Accession# P21781
Expressed Host E. coli
Species Human
Predicted N Terminal His
Molecule Mass The recombinant human KGF consists of 179 amino acids and predicts a molecular mass of 21 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Formulation Lyophilized from sterile 50mM Tris,250mM NaCl,pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Shipping In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
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