BCA-PAK Protein Assay Kit -for Transfection Assay
BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content even in the presence of detergents.
This kit is based on the reduction of Cu2+ to Cu1+ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu1+ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/mL. In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/mL as a protein standard for a convenient preparation of protein concentration standard curves.
Kit contains:
BCA Reagent A (500mL) / Copper Solution B (15mL) / BSA 5x1mL - 1500µg/mL
This kit is based on the reduction of Cu2+ to Cu1+ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu1+ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/mL. In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/mL as a protein standard for a convenient preparation of protein concentration standard curves.
Kit contains:
BCA Reagent A (500mL) / Copper Solution B (15mL) / BSA 5x1mL - 1500µg/mL
| Supplier | Amerigo Scientific |
|---|---|
| Product # | BCA2500 |
| Pricing | 2500 assays, Inquire |