Plant Heat Shock Protein (HSP) ELISA Kit

The ELISA kit applies the double-antibody sandwich method to determine the level of Plant Heat Shock Protein (HSP)in plant samples. Purified Plant Heat Shock Protein (HSP)antibodies are coated onto a microplate to form a solid-phase antibody. To these coated monoclonal antibody wells, Plant Heat Shock Protein (HSP)is added, followed by an HRP-conjugated Plant Heat Shock Protein (HSP)antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added and turns blue under the catalysis of HRP, and then changes to a final yellow color due to the addition of acid. The color intensity is directly proportional to the amount of Plant Heat Shock Protein (HSP)present in the sample. The absorbance (OD value) is measured at 450 nm using an ELISA reader, and the concentration of Plant Heat Shock Protein (HSP)in the plant samples is calculated based on a standard curve.

• Supplier: Lifeasible
• Product #: PEK-158
• specs: 96T/48T