Immobilized Endoproteinase Glu-C on F7m

Endoproteinase Glu-C hydrolyzes peptide and ester linkages specifically at the carboxyl end of glutamic acid (-Glu/-X; in ammonium carbonate pH 7. 8, or ammonium acetate pH 4. 0, buffer A) or of glutamic and aspartic acid (-Glu/-X and -Asp/-X; in phosphate buffer pH 7. 8, buffer B). F7m: 1. 0 mg endoproteinase Glu-C immobilized on matrix F7m per CR-column. 900 units immobilized per CR-column. Nr. 7 Storage buffer: 50 mM Tris/HCl at pH 7. 5, 5 mM EDTA. Nr. 31 Reaction buffer A: 25 mM ammonium acetate, pH 4. 0 (see above)Nr. 32 Washing buffer A: 25 mM ammonium acetate, pH 4. 0, 1 M NaClNr. 62 Reaction buffer B: 50 mM phosphate buffer, pH 7. 8 (see above)Nr. 63 Washing buffer B: 50 mM phosphate buffer, pH 7. 8, 1 M NaCl

• Supplier: Creative Enzymes
• Product #: NATE-1763