[ribosomal protein S12] (aspartate89-C3)-methylthiotransferase

This bacterial enzyme binds two [4Fe-4S] clusters. A bridge of five sulfur atoms is formed between the free Fe atoms of the two [4Fe-4S] clusters. In the first reaction the enzyme transfers a methyl group from AdoMet to the external sulfur ion of the sulfur bridge. In the second reaction the enzyme catalyses the reductive fragmentation of a second molecule of AdoMet, yielding a 5'-deoxyadenosine radical, which then attacks the methylated sulfur atom of the polysulfide bridge, resulting in the transfer of a methylthiol group to aspartate89. The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes.

• Supplier: Creative Enzymes
• Product #: EXWM-3430