NADP / NADPH Assay Kit
NADP+/NADPH Assay Kit is an assay kit designed for the detection and quantification of NADP+/NADPH concentrations in tissue homogenates and cell lysates using a microplate reader.
Detecting total NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfers electrons to WST-8 to produce a yellow reaction product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measuring the OD value at 450 nm.
Detection of NADPH: After treating sample, the sample is heated to decompose any NADP+ in the sample. The remaining NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measuring the OD value at 450 nm.
Detecting NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained in the first two steps as well as the separate content of NADPH.
Detecting total NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfers electrons to WST-8 to produce a yellow reaction product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measuring the OD value at 450 nm.
Detection of NADPH: After treating sample, the sample is heated to decompose any NADP+ in the sample. The remaining NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measuring the OD value at 450 nm.
Detecting NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained in the first two steps as well as the separate content of NADPH.
| Supplier | Abbexa Ltd. |
|---|---|
| Product # | abx096008-96T |
| Pricing | 96 tests USD $536.5 |
| Test Range | 0.02 µmol/L - 5 µmol/L |
| CAS Number | 53-59-8 |
| Availability | Shipped within 5-10 working days. |
| Dry Ice | No |
| Note | This product is for research use only. |