Caspase 8 Substrate 1f, fluorogenic

• Supplier: Creative Peptides
• Product #: A55002
• MolecularWeight: 729.7
• Sequence:
  • One Letter Code: Ac-IETD-AFC
  • Three Letter Code: Ac-Ile-Glu-Thr-Asp-AFC
• Storage: -20°C desiccated and protected from light
• Explanation: AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.
• Reference:
  • 1. Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102;
  • 2. Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30;
  • 3. Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4;
  • 4. Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63.