Phospho-IRS-1 (Ser307) Antibody

• Supplier: CD BioSciences
• Product #: AMA-0015
• Product Category: Antibodies
• Application: WB
• Product Usage Information Dilution: Western Blotting (1:1000)
• Reactivity: Human, Mouse, Rat
• Gene ID: 3667
• MW (Target): 180 kDa
• Storage: Store at -20°C.
• Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser307 of mouse IRS-1 (equivalent to Ser312 of human IRS-1). Antibodies are purified by protein A and peptide affinity chromatography.
• Background: Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.