Recombinant Human TNF Protein

Recombinant Human TNF Protein is produced by E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact bell-shaped homotrimer. The individual subunits have a relative molecular mass of 17484 Daltons each. TNF-α is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

• Supplier: Cohesion Biosciences
• Product #: CRP2398
• Source: E. coli
• Reactivity: Human
• Applications: E, WB, SDS-PAGE, MS
• Delivery Time: Ship in 1 week
• Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
• Gene Symbol: TNF
• Alternative Names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
• Entrez Gene (Human): 7124.0
• SwissProt (Human): P01375
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Quality Control: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage/Stability: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Datasheet: http://www.cohesionbio.com/download/CRP2398.pdf