2'-phosphotransferase
Catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae. The reaction takes place in two steps: in the first step, the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1''-2''-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2'-phosphate. Oligonucleotides with only a terminal 5'- or 3'-phosphate are not substrates.
| Supplier | Creative Enzymes |
|---|---|
| Product # | EXWM-2990 |
| Pricing | 100 ug, contact supplier for pricing |
| CAS | 126905-00-8 |