| Supplier |
CD BioSciences |
| Product # |
AMA-0110 |
| Pricing |
20 μL/100 μL, Inquire
|
| Product Category |
Antibodies
|
| Application |
WB, IP, IHC, IF, F
|
| Product Usage Information Dilution |
Western Blotting (1:1000); Immunoprecipitation (1:100); Immunohistochemistry (1:50 - 1:200); Immunofluorescence (1:100 - 1:400); Flow Cytometry (1:100 - 1:400)
|
| Reactivity |
Human, Monkey, Mouse, Rat
|
| Host |
Rabbit
|
| Gene ID |
2475
|
| UniProt ID |
|
| Source/Isotype |
Rabbit IgG
|
| MW (Target) |
289 kDa
|
| Storage |
Store at -20°C.
|
| Purification |
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR.
|
| Background |
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.
|