Endoglycosidase S (Low Endotoxin) from Streptococcus pyogenes, Recombinant

EndoS LE is an endoglycosidase for deglycosylation of IgG Fc-glycan moieties. IgGZERO(R) LE hydrolyzes Fcglycans on IgG of all human IgG subclasses and IgG from the following species: mouse, rat, monkey, sheep, goat, cow and horse. The enzyme has limited activity on high-mannose and hybrid- type glycans. EndoS LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc on the Fc. EndoS LE is a low endotoxin product,use endotoxin free material and solutions. Physiological reaction conditions at pH 7. 4 and 37°C yields optimal enzyme activity. Other buffers and pH (6-8) are compatible with enzyme activity but the reaction conditions needs to be tested to ensure efficient deglycosylation. EndoS LE has a mass of 110 kDa and contains a His-tag. EndoS LE is for R&D use only.

• Supplier: Creative Enzymes
• Product #: NATE-1780