Ephrin B2 Protein, Mouse, Recombinant (His Tag)

• Supplier: Ace Therapeutics
• Product #: NSC057
• Product Size: 100 µg
• Purity: > 95 % as determined by SDS-PAGE
• Product Type: Proteins
• Synonyms: ELF-2 Protein, Mouse; Epl5 Protein, Mouse; Eplg5 Protein, Mouse; Htk-L Protein, Mouse; LERK-5 Protein, Mouse; Lerk5 Protein, Mouse; NLERK-1 Protein, Mouse
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Activity: Immobilized Ephrin B2 Protein, Mouse, Recombinant (His Tag) at 2 μg/mL (100 μL/well) can bind EphB4 Protein, Mouse, Recombinant (hFc Tag), the EC50 is 3-18 ng/mL.
• Protein Construction: A DNA sequence encoding the mouse EFNB2 (NP_034241.2) extracellular domain (Met 1-Ala 232) was fused with a polyhistidine tag at the C-terminus.
• Accession#: NP_034241.2
• Expressed Host: HEK293 Cells
• Species: Mouse
• Predicted N Terminal: Arg 29
• Molecule Mass: The secreted recombinant mouse EFNB2 comprises 215 amino acids and has a predicted molecular mass of 23.5 kDa. As a result of glycosylation, the apparent molecular mass of rmEFNB2 is approximately 30-40 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.