WesternBright Quantum HRP Substrate
![](https://image.labshake.com/supplier-product/ed33233a-6315-4b47-bd0c-4d9858c889d9/901c2c7e-6cab-4572-8d9c-59a11f2abef7.png)
WesternBright Quantum is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples.
Features Sensitive – detect attomoles of protein per band.
Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
Low background – for high signal to noise
Long lasting signal – image blots hours after substrate incubation
Versatile – Optimized for CCD imaging, and compatible with film detection. In addition, chemifluorescent emissions can be detected with fluorescence imaging systems
Features Sensitive – detect attomoles of protein per band.
Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
Low background – for high signal to noise
Long lasting signal – image blots hours after substrate incubation
Versatile – Optimized for CCD imaging, and compatible with film detection. In addition, chemifluorescent emissions can be detected with fluorescence imaging systems
Supplier | Advansta Corporation |
---|---|
Product # | K-12042-D10 |
Pricing | 2x50 ml (for 1000 cm² of membrane), USD $245.00/ea |
Citations for this product:
-
bioRxiv - Biochemistry 2020Quote: ... ECL detection was performed using WesternBright™ Quantum (K-12042-D10, Advansta) Western blotting substrate and ImageQuant LAS 4000 (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... ECL detection was performed using WesternBrightTM Quantum (K-12042-D10, Advansta) Western blotting substrate and an ImageQuant LAS 4000 (GE Healthcare ...
-
bioRxiv - Genomics 2022Quote: ... Santa Cruz Biotechnology) and HRP substrate (K-12042-D10, Advansta) were used for the western blotting experiments.
-
bioRxiv - Microbiology 2024Quote: ... Enhanced chemiluminescence (ECL) detection was performed using WesternBrightTM Quantum (K-12042-D10, Advansta) Western blotting substrate using ImageQuant LAS 4000 (GE Healthcare ...
-
bioRxiv - Genetics 2024Quote: ... Protein band detection was accomplished using an HRP substrate (Advansta #K-12042-D10) and visualized under a chemiluminescent imaging system ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... The membranes were visualized by G-Box apparatus using chemiluminescent reagent (Advansta, #K-12042-D10). TGX stain-free gels from Bio-Rad (#4568095 ...